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1. WO2020197518 - TETRAHYMENA THERMOPHILA ARTIFICIAL CHROMOSOME 2 (TTAC2) AND ITS USE IN THE RECOMBINANT PROTEIN PRODUCTION

Publication Number WO/2020/197518
Publication Date 01.10.2020
International Application No. PCT/TR2019/051165
International Filing Date 20.12.2019
IPC
C12N 15/63 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
C12N 15/00 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
C07K 14/44 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435from animals; from humans
44from protozoa
CPC
C07K 14/44
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435from animals; from humans
44from protozoa
C12N 15/63
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
C12N 15/79
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
Applicants
  • ANADOLU UNIVERSITESI [TR]/[TR]
Inventors
  • ARSLANYOLU, Muhittin
  • USTUNTANIR DEDE, Ayca Fulya
  • CALISEKI, Mehmet
Agents
  • INVOKAT INTELLECTUAL PROPERTY SERVICES
Priority Data
TR 2019/0429122.03.2019TR
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) TETRAHYMENA THERMOPHILA ARTIFICIAL CHROMOSOME 2 (TTAC2) AND ITS USE IN THE RECOMBINANT PROTEIN PRODUCTION
(FR) CHROMOSOME ARTIFICIEL 2 DE TÉTRAHYMÉNA THERMOPHILA (TTAC2) ET SON UTILISATION DANS LA PRODUCTION DE PROTÉINES RECOMBINANTES
Abstract
(EN)
The present invention relates to Tetrahymena thermophila artificial chromosome (TtAC2) having a nucleotide sequence that is at least 80% homologous to SEQ ID NO: 4 and comprising a modified HSP70.2 promoter having SEQ ID NO: 1, 3´NTS sequence having SEQ ID NO: 2, "5´NTS containing C3 origin, rDNA genes and 3´NTS" sequence having SEQ ID NO: 3. Tetrahymena thermophilaartificial chromosome, which is formed by biomimetics of the Tetrahymena thermophilamacronucleus rDNA minichromosome further comprises a telomere sequence, Neo4 cassette and TtsfGFP marker gene cassette. Within the scope of the present invention, also provided a method for obtaining said Tetrahymena thermophila artificial chromosome. In another aspect, the present invention provides a method for recombinantly producing homologous and/or heterologous genomic DNA, RNA and/or proteins and/or derivatives thereof by using Tetrahymena thermophilaartificial chromosome as a production system/platform. In another aspect, the present invention provides the use of the Tetrahymena thermophila artificial chromosome as a production system/platform for recombinantly producing homologous and/or heterologous genomic DNA, RNA and/or proteins and/or derivatives thereof.
(FR)
La présente invention concerne un chromosome artificiel de tétrahyména thermophila (TtAC2) ayant une séquence nucléotidique qui est homologue d'au moins 80 % à SEQ ID No : 4 et comprenant un promoteur HSP70.2 modifié ayant la SEQ ID No : 1, une séquence 3´NTS ayant la SEQ ID No : 2, "5´NTS contenant une origine C3, les gènes ADNr et la séquence 3´NTS" ayant la SEQ ID No : 3. Le chromosome artificiel de tétrahyména thermophila, qui est formé par biomimétisme du minichromosome de tétrahymena thermophila macronucléus, comprend en outre une séquence de télomère, une cassette de Neo4 et une cassette de gène marqueur TtsfGFP. Dans le cadre de la présente invention, l'invention concerne également un procédé d'obtention dudit chromosome artificiel de tétrahyména thermophila. Dans un autre aspect, la présente invention concerne un procédé de production par recombinaison d'ADN génomique homologue et/ou hétérologue, d'ARN et/ou de protéines et/ou de dérivés de ceux-ci à l'aide d'un chromosome artificiel de tétrahyména thermophila en tant que système/plateforme de production. Dans un autre aspect, la présente invention concerne l'utilisation du chromosome artificiel de tétrahymena thermophila en tant que système/plateforme de production pour produire par recombinaison de l'ADN génomique homologue et/ou hétérologue, de l'ARN et/ou des protéines et/ou des dérivés de ceux-ci.
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