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1. WO2020194141 - STABLE SYSTEM FOR THE IN VITRO CULTURE OF CEREBELLAR GRANULE CELL PRECURSORS (GCP), STABLE METHOD FOR THE IN VITRO CULTURE OF SAID CELLS AND USE OF SAID SYSTEM OR METHOD FOR IN VITRO CULTURE

Publication Number WO/2020/194141
Publication Date 01.10.2020
International Application No. PCT/IB2020/052513
International Filing Date 19.03.2020
IPC
C12N 5/0793 2010.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07Animal cells or tissues
071Vertebrate cells or tissues, e.g. human cells or tissues
079Neural cells
0793Neurons
C12N 5/0797 2010.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07Animal cells or tissues
071Vertebrate cells or tissues, e.g. human cells or tissues
079Neural cells
0797Stem cells; Progenitor cells
CPC
C12N 2501/11
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2501Active agents used in cell culture processes, e.g. differentation
10Growth factors
11Epidermal growth factor [EGF]
C12N 2501/41
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2501Active agents used in cell culture processes, e.g. differentation
40Regulators of development
41Hedgehog proteins; Cyclopamine (inhibitor)
C12N 5/0619
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor;
06Animal cells or tissues; Human cells or tissues
0602Vertebrate cells
0618Cells of the nervous system
0619Neurons
C12N 5/0623
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor;
06Animal cells or tissues; Human cells or tissues
0602Vertebrate cells
0618Cells of the nervous system
0623Stem cells
Applicants
  • UNIVERSITÀ DEGLI STUDI DI ROMA "LA SAPIENZA" [IT]/[IT]
  • FONDAZIONE ISTITUTO ITALIANO DI TECNOLOGIA [IT]/[IT]
Inventors
  • GIANNINI, Giuseppe
  • COLICCHIA, Valeria
  • CAPALBO, Carlo
  • FABRETTI, Francesca
  • DI GIULIO, Stefano
  • BELARDINILLI, Francesca
  • PETRONI, MariaLaura
  • RONCERO, Maria Sahùn
Agents
  • RAIMONDI, Adriana
  • CAVATTONI, Fabio
  • CAVATTONI, Claudia
Priority Data
10201900000437726.03.2019IT
Publication Language English (EN)
Filing Language Italian (IT)
Designated States
Title
(EN) STABLE SYSTEM FOR THE IN VITRO CULTURE OF CEREBELLAR GRANULE CELL PRECURSORS (GCP), STABLE METHOD FOR THE IN VITRO CULTURE OF SAID CELLS AND USE OF SAID SYSTEM OR METHOD FOR IN VITRO CULTURE
(FR) SYSTÈME STABLE DE CULTURE IN VITRO DE PRÉCURSEURS DE CELLULES GRANULEUSES DU CERVEAU (GCP), PROCÉDÉ STABLE DE CULTURE IN VITRO DESDITES CELLULES ET UTILISATION DUDIT SYSTÈME OU PROCÉDÉ DE CULTURE IN VITRO
Abstract
(EN)
This invention relates to an in vitro system for the culture of cerebellar granule cell precursors (granule cell progenitors, GCP) comprising a culture support, mammalian GCP cells and a culture medium comprising at least SAG (Smoothened agonist) and EGF (Epidermal Growth Factor), and a method for the in vitro culture of GCP cells, use of the above-mentioned culture system or method of culture for the generation of in vitro models for study of the pathophysiology of cerebellar granules or use of the above-mentioned culture system or method of culture for use in gene therapy and cell therapy approaches to cerebellar diseases caused by damage or neurodegeneration.
(FR)
La présente invention concerne un système in vitro pour la culture de précurseurs de cellules granuleuses du cerveau (progéniteurs de cellules granuleues, GCP ) comprenant un milieu de culture, des cellules GCP de mammifère et un milieu de culture comprenant au moins SAG (agoniste Smoothened) et EGF (facteur de croissance épidermique), et un procédé de culture in vitro de cellules GCP, l'utilisation du système de culture susmentionné ou du procédé de culture pour la production de modèles pour l'étude de la pathophysiologie des cellules granuleuses du cerveau ou l'utilisation du système de culture mentionné susmentionné ou du procédé de culture pour une utilisation dans une thérapie génique et des dans des approches de thérapie cellulaire pour des maladies cérébelleuses provoquées par une atteinte ou une neurodégénérescence.
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