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1. WO2020194033 - METHOD AND APPARATUS FOR PROCESSING BIOLOGICAL SPECIMENS

Publication Number WO/2020/194033
Publication Date 01.10.2020
International Application No. PCT/IB2019/052479
International Filing Date 27.03.2019
IPC
G01N 1/30 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
1Sampling; Preparing specimens for investigation
28Preparing specimens for investigation
30Staining; Impregnating
G01N 1/31 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
1Sampling; Preparing specimens for investigation
28Preparing specimens for investigation
30Staining; Impregnating
31Apparatus therefor
G01N 1/42 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
1Sampling; Preparing specimens for investigation
28Preparing specimens for investigation
42Low-temperature sample treatment, e.g. cryofixation
G01N 1/44 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
1Sampling; Preparing specimens for investigation
28Preparing specimens for investigation
44Sample treatment involving radiation, e.g. heat
CPC
G01N 1/30
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
1Sampling; Preparing specimens for investigation
28Preparing specimens for investigation ; including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
30Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
G01N 1/31
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
1Sampling; Preparing specimens for investigation
28Preparing specimens for investigation ; including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
30Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
31Apparatus therefor
G01N 1/36
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
1Sampling; Preparing specimens for investigation
28Preparing specimens for investigation ; including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
36Embedding or analogous mounting of samples
Applicants
  • DIAPATH S.P.A. [IT]/[IT]
Inventors
  • BATTISTEL, Alberto
  • LUPO, Carmelo
  • DANZI, Paolo
  • BUSSI, Ottaviano
  • MANENTI, Giorgio
  • CASATTA, Nadia
  • MELONE, Federica
Agents
  • TRUPIANO, Federica
Priority Data
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) METHOD AND APPARATUS FOR PROCESSING BIOLOGICAL SPECIMENS
(FR) PROCÉDÉ ET APPAREIL DE TRAITEMENT D'ÉCHANTILLONS BIOLOGIQUES
Abstract
(EN)
The present invention refers to a method for processing a biological specimen, as well as an apparatus for processing a biological specimen and an airtight cartridge coupable to the above-mentioned apparatus. The method for processing a biological specimen comprises the following steps: physically removing water contained in a fixed biological specimen by evaporation and/or sublimation, including said fixed and dehydrated biological specimen into an embedding medium (paraffin) as well as preferably a further step, which is carried out before dehydrating the sample: treating the biological specimen with fixative vapors, such as formaldehyde vapors. The apparatus of the invention according can comprise: a processing chamber (50); a first chamber (10) for generating fixative vapors such as formaldehyde vapors (40), means for generating a flow of fixative vapors such as formaldehyde vapors from the first chamber to the processing chamber (20), means for physically removing water contained in the biological specimen by evaporation and/or sublimation (70), means for loading and unloading an embedding medium, and an embedding chamber (110).
(FR)
La présente invention concerne un procédé de traitement d'un échantillon biologique, ainsi qu'un appareil de traitement d'un échantillon biologique et une cartouche étanche à l'air pouvant être raccordée à l'appareil susmentionné. Le procédé de traitement d'un échantillon biologique comprend les étapes suivantes : l'élimination physique de l'eau contenue dans un échantillon biologique fixe par évaporation et/ou sublimation, comprenant ledit échantillon biologique fixe et déshydraté dans un milieu d'inclusion (paraffine) ainsi que de préférence une étape supplémentaire, qui est réalisée avant la déshydratation de l'échantillon : le traitement de l'échantillon biologique avec des vapeurs d'agent fixateur, telles que des vapeurs de formaldéhyde. L'appareil de l'invention peut comprendre : une chambre de traitement (50) ; une première chambre (10) destinée à générer des vapeurs d'agent fixateur telles que des vapeurs de formaldéhyde (40), des moyens pour générer un flux de vapeurs d'agent fixateur telles que des vapeurs de formaldéhyde de la première chambre à la chambre de traitement (20), des moyens pour éliminer physiquement l'eau contenue dans l'échantillon biologique par évaporation et/ou sublimation (70), des moyens pour charger et décharger un milieu d'inclusion et une chambre d'inclusion (110).
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