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1. WO2020191797 - METHOD FOR PREPARING UP-CONVERSION LUMINESCENCE FLEXIBLE HYBRID FILM FOR VISUAL DETECTION OF TUMOR MARKERS

Publication Number WO/2020/191797
Publication Date 01.10.2020
International Application No. PCT/CN2019/081160
International Filing Date 03.04.2019
IPC
G01N 21/64 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
63optically excited
64Fluorescence; Phosphorescence
G01N 21/359 2014.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
17Systems in which incident light is modified in accordance with the properties of the material investigated
25Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
31Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
35using infra-red light
359using near infra-red light
G01N 33/48 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
G01N 21/63 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
63optically excited
CPC
G01N 21/359
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
17Systems in which incident light is modified in accordance with the properties of the material investigated
25Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
31Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
35using infra-red light
359using near infra-red light
G01N 21/63
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
63optically excited
G01N 21/64
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
63optically excited
64Fluorescence; Phosphorescence
G01N 33/48
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
Applicants
  • 青岛大学 QINGDAO UNIVERSITY [CN]/[CN]
Inventors
  • 金辉 JIN, Hui
  • 桂日军 GUI, Rijun
  • 孙玉娇 SUN, Yujiao
  • 姜晓文 JIANG, Xiaowen
Agents
  • 青岛高晓专利事务所(普通合伙) QINGDAO GAOXIAO PATENT AGENCY (GENERAL PARTNERSHIP)
Priority Data
201910226383.725.03.2019CN
Publication Language Chinese (ZH)
Filing Language Chinese (ZH)
Designated States
Title
(EN) METHOD FOR PREPARING UP-CONVERSION LUMINESCENCE FLEXIBLE HYBRID FILM FOR VISUAL DETECTION OF TUMOR MARKERS
(FR) PROCÉDÉ DE PRÉPARATION D'UN FILM HYBRIDE SOUPLE DE LUMINESCENCE À CONVERSION ASCENDANTE PERMETTANT LA DÉTECTION VISUELLE DE MARQUEURS TUMORAUX
(ZH) 一种用于肿瘤标志物可视化检测的上转换发光柔性杂化膜的制备方法
Abstract
(EN)
The present invention belongs to the technical field of the preparation of flexible hybrid film materials and probes for visual detection, and particularly relates to a method for preparing an up-conversion luminescence flexible hybrid film for visual detection of tumor markers. Metal-ion-doped black phosphorus quantum dots M-BPQDs are prepared by using ultrasonic and solvothermal processes; mesoporous SiO2 grows on the surfaces thereof and amination modification is performed; the M-BPQDs are connected to a carboxylated single-stranded DNA1; receptor molecules enter holes; single-stranded DNA2 aptamers and the DNA1 are bonded due to base complementation to package receptors in the holes; and an M-BPQD probe is prepared. DNA1 terminal-SH and a composite film formed by assembling polymethyl methacrylate-polyimide-gold nanoparticles in a layer-by-layer manner are bonded by Au-S bonds, and the film and the probe are connected to construct a flexible hybrid film. Compared with the prior art, the flexible hybrid film of the present invention is simple to prepare, low cost and highly sensitive; when a biological fluid sample containing a tumor marker is added in a dropwise manner to the hybrid film, a relationship between the up-conversion luminescence intensity and the concentration of the marker is constructed under the excitation of near-infrared light, so that the visual detection of the marker is realized.
(FR)
La présente invention se rapporte au domaine technique de la préparation de matériaux de film hybrides souples et de sondes permettant une détection visuelle, et concerne en particulier un procédé de préparation d'un film hybride souple de luminescence à conversion ascendante permettant la détection visuelle de marqueurs tumoraux. Des points quantiques de phosphore noir dopé aux ions métalliques M-BPQD sont préparés à l'aide de méthodes ultrasonores et solvothermales ; du SiO 2 mésoporeux croît sur les surfaces de ce dernier et une modification d'amination est effectuée ; les M-BPQD sont reliés à un DNA1 à simple brin carboxylé ; des molécules réceptrices entrent dans des trous ; des aptamères de DNA2 à simple brin et le DNA1 sont liés en raison d'une complémentation de base permettant d'encapsuler des récepteurs dans les trous ; et une sonde M-BPQD est préparée. Le terminal DNA1-SH et un film composite formé par assemblage de nanoparticules de polyméthacrylate de méthyle-polyimide-or couche par couche sont liés par des liaisons Au-S, et le film et la sonde sont reliés pour construire un film hybride souple. Par comparaison avec l'état de la technique, le film hybride souple de la présente invention est simple à préparer, peu coûteux et hautement sensible ; lorsqu'un échantillon de fluide biologique contenant un marqueur tumoral est ajouté goutte à goutte au film hybride, une relation entre l'intensité de luminescence de conversion ascendante et la concentration du marqueur est construite sous l'excitation de la lumière proche infrarouge, de telle sorte que la détection visuelle du marqueur est réalisée.
(ZH)
本发明属于柔性杂化膜材料和可视化检测探针的制备技术领域,具体涉及一种用于肿瘤标志物可视化检测的上转换发光柔性杂化膜的制备方法。采用超声和溶剂热制备金属离子掺杂黑磷量子点M-BPQDs,在其表面生长介孔SiO 2和氨基化改性,连接羧基化单链DNA1,受体分子进入孔内,单链DNA2适体与DNA1因碱基互补结合封装受体于孔内,制得M-BPQDs探针。DNA1终端-SH与聚异丁烯酸甲酯-聚酰亚胺-金纳米粒层层组装复合薄膜以Au-S键结合,连接薄膜与探针构筑柔性杂化膜。与现有技术相比,本发明柔性杂化膜的制备简单,成本低,灵敏度高,当含有肿瘤标志物的生物流体样品滴加到杂化膜上,在近红外光激发下构建上转换发光强度与标志物浓度之间的联系,实现标志物可视化检测。
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