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1. WO2020191480 - BEAD-BASED ANALYSIS OF A SAMPLE

Publication Number WO/2020/191480
Publication Date 01.10.2020
International Application No. PCT/CA2020/050286
International Filing Date 04.03.2020
IPC
G01N 21/77 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
75Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
77by observing the effect on a chemical indicator
G06M 11/00 2006.01
GPHYSICS
06COMPUTING; CALCULATING OR COUNTING
MCOUNTING MECHANISMS; COUNTING OF OBJECTS NOT OTHERWISE PROVIDED FOR
11Counting of objects distributed at random, e.g. on a surface
CPC
G01N 21/77
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
75Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
77by observing the effect on a chemical indicator
G01N 33/5094
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
5005involving human or animal cells
5094for blood cell populations
G01N 33/5302
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
5302Apparatus specially adapted for immunological test procedures
G01N 33/543
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
543with an insoluble carrier for immobilising immunochemicals
G01N 33/54306
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
543with an insoluble carrier for immobilising immunochemicals
54306Solid-phase reaction mechanisms
G01N 33/54313
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
543with an insoluble carrier for immobilising immunochemicals
54313the carrier being characterised by its particulate form
Applicants
  • ALENTIC MICROSCIENCE INC. [CA]/[CA]
Inventors
  • FINE, Alan Marc
Agents
  • SMART & BIGGAR LLP
Priority Data
16/368,70728.03.2019US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) BEAD-BASED ANALYSIS OF A SAMPLE
(FR) ANALYSE FONDÉE SUR DES BILLES D'UN ÉCHANTILLON
Abstract
(EN)
Among other things, two or more different antibodies are caused to bind to one or more units of a chemical component in a sample. Each of the antibodies is attached to one or more beads (e.g., microbeads). The sample is situated on a surface of an image sensor. At the image sensor, light is received originating at a light source that is other than the beads. The received light includes light reflected by, refracted by, or transmitted through the beads. The image sensor captures one or more images of the sample including the beads. At least one of the images of the sample is processed to separately enumerate individual beads and complexes of two or more of the beads attached to the two or more antibodies that are bound to a unit of the chemical component. The results of the processing are used to identify a presence or a level of the chemical component in the sample.
(FR)
L'invention concerne entre autres, au moins deux anticorps différents amenés à se lier à une ou plusieurs unités d'un constituant chimique dans un échantillon. Chacun des anticorps est fixé à une ou plusieurs billes (par exemple, des microbilles). L'échantillon est situé sur une surface d'un capteur d'image. Au niveau du capteur d'image, une lumière est reçue en provenance d'une source de lumière autre que les billes. La lumière reçue comprend une lumière réfléchie par les billes, réfractée par ces dernières ou transmise à travers ces dernières. Le capteur d'image capture une ou plusieurs images de l'échantillon comprenant les billes. Au moins une des images de l'échantillon est traitée afin d'énumérer séparément des billes individuelles et des complexes d'au moins deux des billes fixées auxdits au moins deux anticorps liés à une unité du constituant chimique. Les résultats du traitement sont utilisés pour identifier une présence ou un niveau du constituant chimique dans l'échantillon.
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