WO2020160481 TARGETABLE 3'-OVERHANG NUCLEASE FUSION PROTEINS

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Publication Number WO/2020/160481

Publication Date 06.08.2020

International Application No. PCT/US2020/016229

International Filing Date 31.01.2020

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Applicants

THE GENERAL HOSPITAL CORPORATION [US]/[US]

Inventors

JOUNG, J. Keith
COTTMAN, Rebecca Tayler

Agents

WON, Doyon
DEYOUNG, Janice Kugler

Priority Data

62/800,000		01.02.2019		US
62/908,963		01.10.2019		US

Publication Language English (en)

Filing Language English (EN)

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Title

(EN) TARGETABLE 3'-OVERHANG NUCLEASE FUSION PROTEINS

(FR) PROTÉINES DE FUSION NUCLÉASE À EXTRÉMITÉ SORTANTE 3' POUVANT ÊTRE CIBLÉES

Abstract

(EN) Described herein are zinc finger and dCas9 nuclease fusion proteins and methods of using the same for enhancing repair frequencies at the site of a nuclease-induced double strand breaks (DSB) for use in genome editing.

(FR) L'invention concerne des protéines de fusion nucléase de doigt à zinc et de dCas9 et leurs méthodes d'utilisation pour améliorer des fréquences de réparation au niveau du site d'une rupture de double brin (DSB) induite par une nucléase à des fins d'utilisation dans l'édition génomique.

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N	MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15	Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09	Recombinant DNA-technology
11	DNA or RNA fragments; Modified forms thereof
62	DNA sequences coding for fusion proteins

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N	MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9	Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
14	Hydrolases (3.)
16	acting on ester bonds (3.1)

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N	MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15	Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09	Recombinant DNA-technology
87	Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
90	Stable introduction of foreign DNA into chromosome

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N	MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15	Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09	Recombinant DNA-technology
11	DNA or RNA fragments; Modified forms thereof
62	DNA sequences coding for fusion proteins

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N	MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9	Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
14	Hydrolases (3.)
16	acting on ester bonds (3.1)

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1. WO2020160481 - TARGETABLE 3'-OVERHANG NUCLEASE FUSION PROTEINS

C	CHEMISTRY; METALLURGY
07	ORGANIC CHEMISTRY
K	PEPTIDES
14	Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
195	from bacteria

C	CHEMISTRY; METALLURGY
07	ORGANIC CHEMISTRY
K	PEPTIDES
2319	Fusion polypeptide
80	containing a DNA binding domain, e.g. Lacl or Tet-repressor

C	CHEMISTRY; METALLURGY
07	ORGANIC CHEMISTRY
K	PEPTIDES
2319	Fusion polypeptide
80	containing a DNA binding domain, e.g. Lacl or Tet-repressor