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1. WO2020142598 - ENGINEERED NUCLEASES TO GENERATE MUTATIONS IN PLANTS

Publication Number WO/2020/142598
Publication Date 09.07.2020
International Application No. PCT/US2020/012040
International Filing Date 02.01.2020
IPC
C12N 15/82 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
82for plant cells
C07K 14/41 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
41from lichens
C07K 14/415 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
415from plants
A01H 5/00 2018.01
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
HNEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
5Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
CPC
C07K 14/415
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
415from plants
C12N 15/8247
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
82for plant cells ; , e.g. plant artificial chromosomes (PACs)
8241Phenotypically and genetically modified plants via recombinant DNA technology
8242with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
8243involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
8247involving modified lipid metabolism, e.g. seed oil composition
Applicants
  • CARGILL, INCORPORATED [US]/[US]
Inventors
  • KAMMERZELL, Meridith
Agents
  • PERDOK, Monique M.
  • ARORA, Suneel, USPTO Reg. No. 42,267
  • BEEKMAN, Marvin L., USPTO Reg. No. 38,377
  • BIANCHI, Timothy E., USPTO Reg. No. 39,610
  • BLACK, David W., USPTO Reg. No. 42,331
  • LANG, Allen R., USPTO Reg. No. 58,829
  • MCCRACKIN, Ann M., USPTO Reg. No. 42,858
  • SCHEER, Bradley W., USPTO Reg. No. 47,059
Priority Data
62/788,57804.01.2019US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) ENGINEERED NUCLEASES TO GENERATE MUTATIONS IN PLANTS
(FR) NUCLÉASES MODIFIÉES POUR GÉNÉRER DES MUTATIONS DANS DES PLANTES
Abstract
(EN)
Methods are provided to mutate, in a targeted manner, the genome of a plant cell using a double stranded DNA break inducing enzyme. Also provided are plants, in particular Brassica plants that yield seeds producing oils having a reduced total saturated fatty acid content, and method for making such plants.
(FR)
L'invention concerne des procédés pour muter, de manière ciblée, le génome d'une cellule végétale à l'aide d'une enzyme induisant une rupture d'ADN double brin. L'invention concerne également des plantes, en particulier des plantes de Brassica qui donnent des graines produisant des huiles ayant une teneur réduite en acides gras saturés totaux, et un procédé de production de telles plantes.
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