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1. WO2020142304 - SUPPLEMENTED SERUM-CONTAINING CULTURE MEDIUM FOR ENHANCED ARPE-19 GROWTH AND HUMAN CYTOMEGALOVIRUS VACCINE PRODUCTION

Publication Number WO/2020/142304
Publication Date 09.07.2020
International Application No. PCT/US2019/068230
International Filing Date 23.12.2019
IPC
A01N 1/02 2006.1
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
1Preservation of bodies of humans or animals, or parts thereof
02Preservation of living parts
A61K 35/36 2015.1
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
35Medicinal preparations containing materials or reaction products thereof with undetermined constitution
12Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
36Skin; Hair; Nails; Sebaceous glands; Cerumen; Epidermis; Epithelial cells; Keratinocytes; Langerhans cells; Ectodermal cells
A61K 35/44 2015.1
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
35Medicinal preparations containing materials or reaction products thereof with undetermined constitution
12Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
44Vessels; Vascular smooth muscle cells; Endothelial cells; Endothelial progenitor cells
CPC
A01N 1/02
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES
1Preservation of bodies of humans or animals, or parts thereof
02Preservation of living parts
A61K 35/36
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
35Medicinal preparations containing materials or reaction products thereof with undetermined constitution
12Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
36Skin; Hair; Nails; Sebaceous glands; Cerumen; Epidermis; Epithelial cells; Keratinocytes; Langerhans cells; Ectodermal cells
A61K 35/44
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
35Medicinal preparations containing materials or reaction products thereof with undetermined constitution
12Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
44Vessels; Vascular smooth muscle cells; Endothelial cells; Endothelial progenitor cells
Applicants
  • MERCK SHARP & DOHME CORP. [US]/[US]
  • CHRISTANTI, Sianny [US]/[US] (US)
  • LIM, Esther Wei Yee [US]/[US] (US)
  • RODRIGUEZ, Jason [US]/[US] (US)
  • DANIELS, Christopher, L. [US]/[US] (US)
Inventors
  • CHRISTANTI, Sianny
  • LIM, Esther Wei Yee
  • RODRIGUEZ, Jason
  • DANIELS, Christopher, L.
Common Representative
  • MERCK SHARP & DOHME CORP.
Priority Data
62/787,92903.01.2019US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) SUPPLEMENTED SERUM-CONTAINING CULTURE MEDIUM FOR ENHANCED ARPE-19 GROWTH AND HUMAN CYTOMEGALOVIRUS VACCINE PRODUCTION
(FR) MILIEU DE CULTURE CONTENANT DU SÉRUM ENRICHI POUR LA CROISSANCE D'ARPE -19 ACTIVÉ ET LA PRODUCTION D'UN VACCIN CONTRE LE CYTOMÉGALOVIRUS HUMAIN
Abstract
(EN)
The present invention relates to supplemented serum-containing cell culture media that provides enhances ARPE-19 cell growth and/or improves the yield of human cytomegalovirus (HCMV) grown in ARPE-19 cell cultures. The media of the invention includes two additives, a hormone (e.g., a glucocorticoid hormone such as dexamethasone) and a growth factor (e.g., EGF). The invention further provides methods of producing HCMV in such growth media.
(FR)
La présente invention concerne des milieux de culture cellulaire enrichis contenant du sérum qui permettent d'activer la croissance des cellules ARPE -19 et/ou d'améliorer le rendement du cytomégalovirus humain (HCMV) cultivé dans des cultures de cellules ARPE -19. Le milieu de l'invention comprend deux additifs, une hormone (par exemple, une hormone glucocorticoïde telle que la dexaméthasone) et un facteur de croissance (par exemple, EGF). L'invention concerne en outre des méthodes de production du HCMV dans de tels milieux de culture.
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