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1. WO2020141143 - ONE POT SYNTHESIS OF SETS OF OLIGONUCLEOTIDES

Publication Number WO/2020/141143
Publication Date 09.07.2020
International Application No. PCT/EP2019/087048
International Filing Date 26.12.2019
IPC
C12Q 1/6806 2018.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction assay
CPC
C12Q 1/6806
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
C12Q 1/6844
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6844Nucleic acid amplification reactions
Applicants
  • DNA SCRIPT [FR]/[FR]
Inventors
  • GODRON, Xavier
  • HORGAN, Adrian
Agents
  • CABINET BECKER ET ASSOCIES
Priority Data
19305007.703.01.2019EP
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) ONE POT SYNTHESIS OF SETS OF OLIGONUCLEOTIDES
(FR) SYNTHÈSE MONOTOPE D'ENSEMBLES D'OLIGONUCLÉOTIDES
Abstract
(EN)
The invention is directed to methods for synthesizing a plurality of oligonucleotides in the same reaction vessel, and in some embodiments, using the synthesized oligonucleotides in an oligonucleotide-based assay in such reaction vessel. In some embodiments, methods of the invention are implemented by steps of (a) providing a plurality of different initiators attached to one or more supports, each different initiator having a terminal nucleotide with a different 3'-O-blocking group; (b) for each different initiator, synthesizing a polynucleotide by repeated cycles of template-free enzymatic additions of 3'-O-blocked nucleoside triphosphates, wherein the blocking group of the 3'-O-blocked nucleoside triphosphate is removable under deblocking conditions orthogonal to the deblocking conditions for removing blocking groups of the other initiators; and (c) releasing the oligonucleotides from the polynucleotides and the one or more solid supports.
(FR)
L'invention concerne des procédés de synthèse d'une pluralité d'oligonucléotides dans la même cuve de réaction, et dans certains modes de réalisation, l'utilisation des oligonucléotides synthétisés dans un dosage à base d'oligonucléotides dans un tel récipient de réaction. Dans certains modes de réalisation, les procédés de l'invention sont mis en oeuvre par les étapes consistant à (a) fournir une pluralité d'initiateurs différents attachés à un ou plusieurs Supports, chaque initiateur différent ayant un nucléotide terminal avec un groupe 3 '-O-bloquant différent; (b) pour chaque initiateur différent, synthétiser un polynucléotide par cycles répétés d'additions enzymatiques sans matrice de nucléosides triphosphates 3 '-O-bloqués, le groupe de blocage du triphosphate de nucléoside 3 '-O-bloqué pouvant être retiré dans des conditions de déblocage orthogonales aux conditions de déblocage pour éliminer les groupes de blocage des autres initiateurs; et (c) libérer les oligonucléotides des polynucléotides et l'ou les supports solides.
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