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1. WO2020141062 - MODULATION OF NITRATE LEVELS IN PLANTS VIA MUTATION OF NITRATE REDUCTASE

Publication Number WO/2020/141062
Publication Date 09.07.2020
International Application No. PCT/EP2019/085321
International Filing Date 16.12.2019
IPC
A01H 5/12 2018.01
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
HNEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
5Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
12Leaves
A01H 6/82 2018.01
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
HNEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
6Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
82Solanaceae, e.g. pepper, tobacco, potato, tomato or eggplant
C12N 9/06 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
02Oxidoreductases (1.), e.g. luciferase
06acting on nitrogen containing compounds as donors (1.4, 1.5, 1.7)
A24B 15/00 2006.01
AHUMAN NECESSITIES
24TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
BMANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
15Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
CPC
A01H 5/12
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
HNEW PLANTS OR ; NON-TRANSGENIC; PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
5Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
12Leaves
A01H 6/823
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
HNEW PLANTS OR ; NON-TRANSGENIC; PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
6Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
82Solanaceae, e.g. pepper, tobacco, potato, tomato or eggplant
823Nicotiana, e.g. tobacco
A24B 15/00
AHUMAN NECESSITIES
24TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
BMANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
15Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
C12N 9/0044
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes; Proenzymes; Compositions thereof
0004Oxidoreductases (1.)
0012acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
0044acting on other nitrogen compounds as donors (1.7)
Applicants
  • PHILIP MORRIS PRODUCTS S.A. [CH]/[CH]
Inventors
  • BOVET, Lucien
  • CAMPANONI, Prisca
  • GOEPFERT, Simon
Agents
  • SCRIPT IP LIMITED
  • LLOYD, Dr John Scott
Priority Data
18215913.730.12.2018EP
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) MODULATION OF NITRATE LEVELS IN PLANTS VIA MUTATION OF NITRATE REDUCTASE
(FR) MODULATION DE NIVEAUX DE NITRATE DANS DES PLANTES PAR MUTATION DE NITRATE RÉDUCTASE
Abstract
(EN)
A plant cell comprising: (a) a polynucleotide sequence encoding a nitrate reductase polypeptide comprising a contiguous polypeptide sequence of SEQ ID NO: 5 or SEQ ID NO: 7, wherein methionine is substituted for an amino acid that reduces nitrate reductase activity in the plant cell as compared to a control plant cell; (b) a polypeptide sequence encoded by the polynucleotide sequence set forth in (a); or (c) a construct, vector or expression vector comprising the polynucleotide sequence set forth in (b).
(FR)
L'invention concerne une cellule végétale comprenant : (a) une séquence polynucléotidique codant pour un polypeptide de nitrate réductase comprenant une séquence polypeptidique contiguë de SEQ ID NO : 5 ou SEQ ID NO : 7, la méthionine étant substituée pour un acide aminé qui réduit l'activité de nitrate réductase dans la cellule végétale par rapport à une cellule végétale témoin; (b) une séquence polypeptidique codée par la séquence polynucléotidique présentée au point (a); ou (c) une construction, un vecteur ou un vecteur d'expression comprenant la séquence polynucléotidique présentée au point (b).
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