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1. WO2020140693 - GENE TARGET REGION ENRICHMENT METHOD AND KIT

Publication Number WO/2020/140693
Publication Date 09.07.2020
International Application No. PCT/CN2019/123891
International Filing Date 09.12.2019
IPC
C12Q 1/6806 2018.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction assay
CPC
C12Q 1/6806
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
Applicants
  • 上海臻迪基因科技有限公司 SHANGHAI ZENISIGHT LTD. [CN]/[CN]
Inventors
  • 郭志伟 GUO, Zhiwei
  • 李英辉 LI, Yinghui
  • 陈倩 CHEN, Qian
  • 胡荣君 HU, Rongjun
Agents
  • 北京卓岚智财知识产权代理事务所(特殊普通合伙) BEIJING ZHUOLANIP INTELLECTUAL PROPERTY AGENT FIRM
Priority Data
201910002408.502.01.2019CN
201910897689.520.09.2019CN
Publication Language Chinese (ZH)
Filing Language Chinese (ZH)
Designated States
Title
(EN) GENE TARGET REGION ENRICHMENT METHOD AND KIT
(FR) PROCÉDÉ ET KIT D'ENRICHISSEMENT DE RÉGION CIBLE DE GÈNE
(ZH) 基因目标区域富集方法及试剂盒
Abstract
(EN)
Provided is a gene target region enrichment method and a kit. The method comprises (1) amplifying fragmented DNA comprising a target region by means of a specific probe so as to obtain a captured extension product, wherein the specific probe comprises a sequence complementary to the target region of the fragmented DNA, and the 3' terminal nucleotide of the specific probe is modified to prevent a ligation reaction at the 3' terminal of the specific probe; and (2) linking the 3' terminal of the captured extension product obtained in step (1) to linker DNA to obtain a ligation product.
(FR)
Procédé et kit d'enrichissement de région cible de gène. Le procédé comprend (1) l'amplification d'ADN fragmenté comprenant une région cible au moyen d'une sonde spécifique de façon à obtenir un produit d'extension capturé, la sonde spécifique comprenant une séquence complémentaire de la région cible de l'ADN fragmenté, et le nucléotide 3' terminal de la sonde spécifique étant modifié pour empêcher une réaction de ligature au niveau du 3' terminal de la sonde spécifique; et (2) la liaison du 3'terminal du produit d'extension capturé obtenu à l'étape (1) à l'ADN de liaison pour obtenir un produit de ligature.
(ZH)
提供了一种基因目标区域富集方法及试剂盒。所述方法包括:(1)通过特异性探针扩增包括目标区域的片段化DNA,以提供捕获延伸产物,所述的特异性探针包括与所述片段化DNA的目标区域互补的序列,所述特异性探针的3'末端核苷酸被修饰,用于阻止所述特异性探针的3'末端发生连接反应;(2)将步骤(1)所提供的捕获延伸产物的3'末端连接接头DNA,以提供连接产物。
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