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1. WO2020140035 - METHODS OF PREPARING SAMPLES FOR PROTEOMIC ANALYSIS

Publication Number WO/2020/140035
Publication Date 02.07.2020
International Application No. PCT/US2019/068743
International Filing Date 27.12.2019
IPC
G01N 33/68 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68involving proteins, peptides or amino acids
G01N 30/72 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
30Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography
02Column chromatography
62Detectors specially adapted therefor
72Mass spectrometers
G01N 30/88 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
30Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography
02Column chromatography
88Integrated analysis systems specially adapted therefor, not covered by a single one of groups G01N30/04-G01N30/86146
CPC
G01N 2030/8831
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
30Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography ; or field flow fractionation;
02Column chromatography
88Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
8809analysis specially adapted for the sample
8813biological materials
8831involving peptides or proteins
G01N 33/6848
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68involving proteins, peptides or amino acids
6803General methods of protein analysis not limited to specific proteins or families of proteins
6848Methods of protein analysis involving mass spectrometry
Applicants
  • STRECK, INC. [US]/[US]
Inventors
  • SOBANSKY, Matthew
Agents
  • HONG, Julie, J.
Priority Data
62/785,50127.12.2018US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) METHODS OF PREPARING SAMPLES FOR PROTEOMIC ANALYSIS
(FR) PROCÉDÉS DE PRÉPARATION D'ÉCHANTILLONS POUR UNE ANALYSE PROTÉOMIQUE
Abstract
(EN)
Provided herein are methods of preparing a protein sample for proteomic analysis. In exemplary embodiments, the method comprises (a) contacting a blood sample comprising proteins with a protective agent comprising an anticoagulant (AC) and an aldehyde releaser (AR), to obtain a mixture, optionally, wherein the blood sample is added to a blood collection tube (BCT) comprising the protective agent, and (b) isolating a fraction comprising proteins or a source of proteins from the mixture to yield a protein sample or a source of a protein sample, wherein steps of the method are carried out in the absence of exogenous proteolytic enzyme inhibitors, wherein the protein sample is suitable for proteomic analysis. Preferably, the protective agent consists of essentially (i) about 300 g/l to about 700 g/l imidazolidinyl urea; (ii) about 20 g/l to about 60 g/l glycine; and (iii) about 60 g/l to about 100 g/l EDTA; and the the protein sample is analysed via mass spectrometry-based proteomic methods.
(FR)
La présente invention concerne des procédés de préparation d'un échantillon de protéine pour une analyse protéomique. Dans des modes de réalisation donnés à titre d'exemple, le procédé consiste (a) à mettre en contact un échantillon de sang comprenant des protéines avec un agent de protection comprenant un anticoagulant (AC pour AntiCoagulant) et un dispositif de libération d'aldéhyde (AR pour Aldehyde Releaser) pour obtenir un mélange, facultativement, l'échantillon de sang étant ajouté à un tube de collecte de sang (BCT pour Blood Collection Tube) comprenant l'agent de protection, et (b) à isoler une fraction comprenant des protéines ou une source de protéines du mélange pour produire un échantillon de protéine ou une source d'un échantillon de protéine, les étapes du procédé étant effectuées en l'absence d'inhibiteurs d'enzyme protéolytique exogènes, l'échantillon de protéine étant approprié pour une analyse protéomique. De préférence, l'agent de protection est constitué essentiellement (i) entre environ 300 g/l et environ 700 g/l d'imidazolidinyl urée; (ii) entre environ 20 g/l et environ 60 g/l de glycine; et (iii) entre environ 60 g/l et environ 100 g/l d'EDTA; et l'échantillon de protéine est analysé par le biais de procédés protéomiques basés sur une spectrométrie de masse.
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