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1. WO2020139629 - VISUALIZATION METHOD FOR ANALYZING ORAL BIOFILM GROWTH

Publication Number WO/2020/139629
Publication Date 02.07.2020
International Application No. PCT/US2019/066903
International Filing Date 17.12.2019
IPC
G01N 21/64 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
63optically excited
64Fluorescence; Phosphorescence
G02B 21/00 2006.01
GPHYSICS
02OPTICS
BOPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
21Microscopes
G02B 21/24 2006.01
GPHYSICS
02OPTICS
BOPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
21Microscopes
24Base structure
CPC
C12Q 1/06
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
02involving viable microorganisms
04Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
06Quantitative determination
G01B 11/06
GPHYSICS
01MEASURING; TESTING
BMEASURING LENGTH, THICKNESS OR SIMILAR LINEAR DIMENSIONS; MEASURING ANGLES; MEASURING AREAS; MEASURING IRREGULARITIES OF SURFACES OR CONTOURS
11Measuring arrangements characterised by the use of optical means
02for measuring length, width or thickness
06for measuring thickness ; ; e.g. of sheet material
G01N 2021/8433
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
84Systems specially adapted for particular applications
8422Investigating thin films, e.g. matrix isolation method
8427Coatings
8433Comparing coated/uncoated parts
G01N 21/6458
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
63optically excited
64Fluorescence; Phosphorescence
645Specially adapted constructive features of fluorimeters
6456Spatial resolved fluorescence measurements; Imaging
6458Fluorescence microscopy
G01N 21/8422
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
84Systems specially adapted for particular applications
8422Investigating thin films, e.g. matrix isolation method
G02B 21/0076
GPHYSICS
02OPTICS
BOPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
21Microscopes
0004specially adapted for specific applications
002Scanning microscopes
0024Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
0052Optical details of the image generation
0076arrangements using fluorescence or luminescence
Applicants
  • COLGATE-PALMOLIVE COMPANY [US]/[US]
Inventors
  • SARMA, Tulika
  • BEGUM-GAFUR, Rehana
Agents
  • ST. MARTIN, Anne, Louise
  • WILKINS, Clifford E.
Priority Data
62/784,92226.12.2018US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) VISUALIZATION METHOD FOR ANALYZING ORAL BIOFILM GROWTH
(FR) PROCÉDÉ DE VISUALISATION POUR ANALYSER LA CROISSANCE D'UN BIOFILM BUCCAL
Abstract
(EN)
Methods for visualizing oral biofilm growth and methods for identifying compounds that modulate biofilm growth are provided. Biofilm samples are stained with fluorescent dyes and viewed with a confocal laser scanning microscope. Biofilm samples are in a container with a cover slip configured for viewing the biofilm sample with the confocal laser scanning microscope and the coverslip has a thickness variation of 0.02 mm or less.
(FR)
L'invention concerne des procédés de visualisation de la croissance de biofilm buccal et des procédés d'identification de composés qui modulent la croissance de biofilm. Des échantillons de biofilm sont colorés avec des colorants fluorescents et visualisés avec un microscope confocal à balayage laser. Des échantillons de biofilm sont dans un récipient avec une lamelle de recouvrement conçue pour visualiser l'échantillon de biofilm avec le microscope confocal à balayage laser et la lamelle couvre-objet a une variation d'épaisseur inférieur ou égal à 0,02 mm.
Also published as
Latest bibliographic data on file with the International Bureau