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1. WO2020135712 - METHOD FOR GROWING ARUNDO DONAX PLANT

Publication Number WO/2020/135712
Publication Date 02.07.2020
International Application No. PCT/CN2019/129195
International Filing Date 27.12.2019
Chapter 2 Demand Filed 28.10.2020
IPC
A01H 4/00 2006.01
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
HNEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
4Plant reproduction by tissue culture techniques
A01H 1/08 2006.01
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
HNEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
1Processes for modifying genotypes
06Processes for producing mutations, e.g. treatment with chemicals or with radiation
08Methods or apparatus for producing changes in chromosome number
A01H 1/02 2006.01
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
HNEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
1Processes for modifying genotypes
02Methods or apparatus for hybridisation; Artificial pollination
CPC
A01H 1/02
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
HNEW PLANTS OR ; NON-TRANSGENIC; PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
1Processes for modifying genotypes ; ; Plants characterised by associated natural traits;
02Methods or apparatus for hybridisation; Artificial pollination ; ; Fertility
A01H 1/08
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
HNEW PLANTS OR ; NON-TRANSGENIC; PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
1Processes for modifying genotypes ; ; Plants characterised by associated natural traits;
06Processes for producing mutations, e.g. treatment with chemicals or with radiation
08Methods for producing changes in chromosome number
A01H 4/00
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
HNEW PLANTS OR ; NON-TRANSGENIC; PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
4Plant reproduction by tissue culture techniques ; ; Tissue culture techniques therefor
Applicants
  • 武汉兰多生物科技有限公司 WUHAN RUNDO BIOTECHNOLOGY CO. LTD [CN]/[CN]
Inventors
  • 戴军 DAI, Jun
  • 韩晓红 HAN, Xiaohong
Agents
  • 北京景闻知识产权代理有限公司 JW & PARTNERS
Priority Data
201811619497.X28.12.2018CN
201811619500.828.12.2018CN
201811619518.828.12.2018CN
Publication Language Chinese (ZH)
Filing Language Chinese (ZH)
Designated States
Title
(EN) METHOD FOR GROWING ARUNDO DONAX PLANT
(FR) PROCÉDÉ DE CULTURE DE PLANTE ARUNDO DONAX
(ZH) 培育芦竹植株的方法
Abstract
(EN)
A method for growing an Arundo donax plant, comprising the following steps: S1: taking off a flower bud in the mononucleate microspore stage on an Arundo donax having great resistance and of a large plant size, then disinfecting, and retrieving the anther from the disinfected flower bud under a dissecting microscope; S2: inoculating the anther obtained in step S1 on a callus culture medium for callus-induced culturing; S3: inoculating the Arundo donax callus obtained in step S2 onto an adventitious bud differentiation culture medium for differentiated culturing to acquire an Arundo donax adventitious bud; inoculating the Arundo donax adventitious bud obtained in step S3 on a rooting culture medium for rooting culturing; and S5: identifying the ploidy of the product obtained in step S4 to acquire an Arundo donax haploid plant.
(FR)
L'invention concerne un procédé de culture de plante Arundo donax, lequel comprend les étapes consistant à : S1 : prélever un bourgeon de fleur au stade de microspore mononucléée sur une plante Arundo donax ayant une grande résistance et une grande taille de plante, puis désinfecter et récupérer l'anthère à partir du bourgeon de fleur désinfecté sous stéréomicroscope ; S2 : inoculer l'anthère obtenue à l'étape S1 sur un milieu de culture de cals pour une culture induite par cals ; S3 : inoculer le cal d’Arundo donax obtenu à l'étape S2 sur un milieu de culture à différenciation de bourgeon adventif pour une culture différenciée afin d’obtenir un bourgeon adventif d'Arundo donax ; l'inoculation du bourgeon adventif d'Arundo donax obtenu à l'étape S3 sur un milieu de culture d'enracinement pour la culture d'enracinement ; et S5 : identifier la ploïdie du produit obtenu à l'étape S4 pour obtenir une plante haploïde Arundo donax.
(ZH)
培育芦竹单倍体植株的方法,包括下列步骤:S1:将抗逆性好、植株大的芦竹上处于单核靠边期的花芽取下,然后进行消毒处理,将消毒处理后的花芽在解剖镜下取出花药;S2:将步骤S1中得到的所述花药接种在愈伤组织培养基上进行愈伤组织诱导培养;S3:将步骤S2中获得的所述芦竹愈伤组织接种于不定芽分化培养基上进行分化培育,获得芦竹不定芽;S4:将步骤S3中得到的所述芦竹不定芽接种于生根培养基上进行生根培养;S5:对步骤S4所得到的产物倍性鉴定,以便获得芦竹单倍体植株。
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