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1. WO2020135549 - MEDIUM FOR INDUCING PLASMID COPY NUMBER INCREASE AND APPLICATION THEREOF

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Publication Number WO/2020/135549
Publication Date 02.07.2020
International Application No. PCT/CN2019/128523
International Filing Date 26.12.2019
IPC
C12N 1/20 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
20Bacteria; Culture media therefor
C12N 1/21 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
20Bacteria; Culture media therefor
21modified by introduction of foreign genetic material
C12R 1/19 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C-C12Q75
1Microorganisms
01Bacteria or actinomycetales
185Escherichia
19Escherichia coli
CPC
C12N 1/20
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1Microorganisms, e.g. protozoa; Compositions thereof
20Bacteria
C12N 15/70
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
70Vectors or expression systems specially adapted for E. coli
C12R 1/19
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
RPROCESSES USING MICROORGANISMS
1Processes using microorganisms
01using bacteria or actinomycetales
185Escherichia
19Escherichia coli
Applicants
  • 江苏金斯瑞生物科技有限公司 JIANGSU GENSCRIPT BIOTECH CO., LTD. [CN]/[CN]
Inventors
  • 赵方龙 ZHAO, Fanglong
  • 马艳秋 MA, Yanqiu
  • 王嫚 WANG, Man
  • 刘凡 LIU, Fan
  • 吴政宪 WU, Cheng-Hsien
Agents
  • 北京集佳知识产权代理有限公司 UNITALEN ATTORNEYS AT LAW
Priority Data
201811610822.627.12.2018CN
Publication Language Chinese (ZH)
Filing Language Chinese (ZH)
Designated States
Title
(EN) MEDIUM FOR INDUCING PLASMID COPY NUMBER INCREASE AND APPLICATION THEREOF
(FR) SUPPORT POUR INDUIRE UNE AUGMENTATION DU NOMBRE DE COPIES DE PLASMIDE ET APPLICATION CORRESPONDANTE
(ZH) 诱导质粒拷贝数增加的培养基及其应用
Abstract
(EN)
Disclosed is a medium for inducing plasmid copy number increase and an application thereof. Compared to conventional plasmid copy number induction methods, the medium increases plasmid extraction concentration by 45-95%. Compared to the induction method in which a medium is used without adding glucose and arabinose, plasmid extraction concentration is increased by 110-440%. The medium plays an important role in inducing the increase in plasmid copy number and achieving high-throughput production.
(FR)
L'invention concerne un support pour induire une augmentation du nombre de copies de plasmide et une application correspondante. Par rapport aux procédés classiques d'induction de nombre de copies de plasmide, le support augmente la concentration d'extraction de plasmide de 45 à 95 %. Par rapport au procédé d'induction dans lequel un support est utilisé sans ajout de glucose et d'arabinose, la concentration d'extraction de plasmide est augmentée de 110 à 440 %. Le support joue un rôle important dans l'induction de l'augmentation du nombre de copies de plasmide et la réalisation d'une production à haut rendement.
(ZH)
一种诱导质粒拷贝数增加的培养基及其应用，该培养基相比于传统质粒拷贝数诱导方法，质粒抽提浓度提高了45-95％，相比于不加葡萄糖与阿拉伯糖的培养基诱导方法，质粒抽提浓度提高了110-440％，该培养基在诱导质粒拷贝数增加以及实现高通量生产中起到很重要的作用。
Also published as
CN111378609
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