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1. WO2020135108 - DUCK PLAGUE VIRUS GE AND GI DUAL-GENE TRACELESS DELETION STRAIN DPV CHV-DELTA GE + DELTA GI AND CONSTRUCTION METHOD THEREFOR

Publication Number WO/2020/135108
Publication Date 02.07.2020
International Application No. PCT/CN2019/125291
International Filing Date 13.12.2019
IPC
C12N 7/01 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
7Viruses, e.g. bacteriophages; Compositions thereof; Preparation or purification thereof
01Viruses, e.g. bacteriophages, modified by introduction of foreign genetic material
C12N 15/63 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
C12R 1/93 2006.01
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C-C12Q75
1Microorganisms
92Viruses
93Animal viruses
CPC
C07K 14/005
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07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
005from viruses
C12N 15/63
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
C12N 2710/16321
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2710dsDNA Viruses
00011dsDNA Viruses
16011Herpesviridae
16311Mardivirus, e.g. Gallid herpesvirus 2, Marek-like viruses, turkey HV
16321Viruses as such, e.g. new isolates, mutants or their genomic sequences
C12N 2710/16322
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2710dsDNA Viruses
00011dsDNA Viruses
16011Herpesviridae
16311Mardivirus, e.g. Gallid herpesvirus 2, Marek-like viruses, turkey HV
16322New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
C12N 2800/204
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2800Nucleic acids vectors
20Pseudochromosomes, minichrosomosomes
204of bacterial origin, e.g. BAC
C12N 2800/30
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2800Nucleic acids vectors
30Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
Applicants
  • 四川农业大学 SICHUAN AGRICULTURAL UNIVERSITY [CN]/[CN]
Inventors
  • 程安春 CHENG, Anchun
  • 刘田 LIU, Tian
  • 汪铭书 WANG, Mingshu
Agents
  • 成都正华专利代理事务所 CHENGDU ZHENGHUA PATENT AGENCY
Priority Data
201811599537.926.12.2018CN
Publication Language Chinese (ZH)
Filing Language Chinese (ZH)
Designated States
Title
(EN) DUCK PLAGUE VIRUS GE AND GI DUAL-GENE TRACELESS DELETION STRAIN DPV CHV-DELTA GE + DELTA GI AND CONSTRUCTION METHOD THEREFOR
(FR) GÈNE GE DU VIRUS DE LA PESTE DU CANARD ET SOUCHE À DÉLETION SANS TRACE DE GÈNE DOUBLE GI DPV CHV-DELTA GE + GÉNE GI DELTA ET PROCÉDÉ DE CONSTRUCTION ASSOCIÉ
(ZH) 鸭瘟病毒gE和gI双基因无痕缺失株DPV CHv-ΔgE+ΔgI及其构建方法
Abstract
(EN)
Provided are a duck plague virus gE and gI dual-gene traceless deletion strain DPV CHv-delta gE + delta gI and a construction method therefor. A duck plague virus gE gene and a duck plague virus gI gene are deleted by means of two times of homologous recombination on a bacterial artificial chromosome recombination duck plague virus rescue system platform by using a GS1783 escherichia coli strain and a pEPkan-S plasmid, a MiniF element is deleted by using an intracellular spontaneous homologous recombination method, and thus the construction of a duck plague virus dual-gene traceless deletion strain free from exogenous base and MiniF element residue is completed for the first time.
(FR)
L'invention concerne un gène gE du virus de la peste du canard et une souche à délétion sans trace de gène double gI DPV CHv-delta gE + gène gI delta et un procédé de construction associé. Un gène gE du virus de la peste du canard et un gène gI de virus peste de canard sont supprimés au moyen de deux moments de recombinaison homologue, sur une plateforme de système de sauvetage du virus de la peste du canard de recombinaison de chromosome artificiel bactérien, à l'aide d'une souche d'Escherichia coli GS1783 et d'un plasmide pEPkan-S; un élément MiniF est supprimé à l'aide d'un procédé de recombinaison homologue spontanée intracellulaire, et ainsi la construction d'une souche à délétion sans trace du virus de la peste du canard de gène double exempte de base exogène et de résidu d'élément MiniF est achevée pour la première fois.
(ZH)
提供了一种鸭瘟病毒gE和gI双基因无痕缺失株DPV CHv-ΔgE+ΔgI及其构建方法。利用GS1783大肠杆菌菌株及pEPkan-S质粒,在细菌人工染色体重组鸭瘟病毒拯救系统平台上经两次同源重组缺失鸭瘟病毒gE基因和gI基因,再经过细胞内自发同源重组方法缺失MiniF元件,首次完成了无外源碱基和MiniF元件残留的鸭瘟病毒双基因无痕缺失株的构建。
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