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1. WO2020134688 - METHOD FOR PREPARING HIGH-PURITY HERICIUM ERINACEUS POLYSACCHARIDE BY FERMENTING HERICIUM ERINACEUS, AND FERMENTATION MEDIUM THEREOF

Publication Number WO/2020/134688
Publication Date 02.07.2020
International Application No. PCT/CN2019/118947
International Filing Date 15.11.2019
IPC
C12P 19/04 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
19Preparation of compounds containing saccharide radicals
04Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
C12R 1/645 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C-C12Q75
1Microorganisms
645Fungi
CPC
C12P 19/04
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
19Preparation of compounds containing saccharide radicals
04Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
Applicants
  • 华熙生物科技股份有限公司 BLOOMAGE BIOTECHNOLOGY CORPORATION LIMITED [CN]/[CN]
  • 山东华熙海御生物医药有限公司 SHANDONG BLOOMAGE HYINC BIOPHARM CORPORATION LIMITED [CN]/[CN]
Inventors
  • 贾玉倩 JIA, Yuqian
  • 陆震 LU, Zhen
  • 魏玉洁 WEI, Yujie
  • 杨旭 YANG, Xu
  • 孙元军 SUN, Yuanjun
  • 石艳丽 SHI, Yanli
  • 郭学平 GUO, Xueping
  • 栾贻宏 LUAN, Yihong
Agents
  • 北京市中咨律师事务所 ZHONGZI LAW OFFICE
Priority Data
201811605005.126.12.2018CN
Publication Language Chinese (ZH)
Filing Language Chinese (ZH)
Designated States
Title
(EN) METHOD FOR PREPARING HIGH-PURITY HERICIUM ERINACEUS POLYSACCHARIDE BY FERMENTING HERICIUM ERINACEUS, AND FERMENTATION MEDIUM THEREOF
(FR) PROCÉDÉ DE PRÉPARATION DE POLYSACCHARIDE DE HERICIUM ERINACEUS DE HAUTE PURETÉ PAR FERMENTATION DE HERICIUM ERINACEUS, ET MILIEU DE FERMENTATION CORRESPONDANT
(ZH) 一种发酵猴头菌制备高纯度猴头菌多糖的方法和发酵培养基
Abstract
(EN)
Disclosed is a method for preparing a high-purity Hericium erinaceus polysaccharide by fermenting Hericium erinaceus, wherein same falls within the technical field of microorganisms. The method comprises the following steps: inoculating a liquid seed medium with a Hericium erinaceus strain and culturing same to a log phase; transferring the seeds to a fermentation medium and adding glass beads to disperse the thallus; at the same time, adding a coenzyme to promote the transformation and control the pH value, temperature and carbon source concentration; putting same on a shaker for fermentation under shaking; subjecting the fermented broth to enzymolysis to allow the mycelia to form single protoplast wall-breaking cells; and performing heating, decolorization, filtering, ultrafiltration, repeated alcohol precipitation and drying precipitates to obtain the high-purity Hericium erinaceus polysaccharide, wherein the purity of the polysaccharide can reach at least 90%, and the content thereof is approximately 5 to 6 g/L.
(FR)
Procédé de préparation d'un polysaccharide d'Hericium erinaceus de haute pureté par fermentation d'Hericium erinaceus. Le procédé comprend les étapes suivantes : inoculation d'un milieu de graine liquide avec une souche de Hericium erinaceus et sa la culture dans une phase logarithmique; transfert des graines vers un milieu de fermentation et ajout de billes de verre pour disperser le thallus; simultanément, ajout d'une coenzyme pour favoriser la transformation et la régulation de la valeur de pH, la température et la concentration de source de carbone; sa la mise en place sur un agitateur pour la fermentation sous agitation; soumettre le bouillon fermenté à une enzymolyse pour permettre au mycélium de former des cellules protoplastes à rupture de paroi uniques; et effectuer un chauffage, une décoloration, un filtrage, une ultrafiltration, des précipités d'alcool répétés et des précipités de séchage pour obtenir le polysaccharide d'Hericium erinaceus de haute pureté, la pureté du polysaccharide pouvant atteindre au moins 90 %, et sa teneur étant d'environ 5 À 6 g/L
(ZH)
一种发酵猴头菌制备高纯度猴头菌多糖的方法,属于微生物技术领域。所述方法包括如下:将猴头菌菌株接种到液体种子培养基培养至对数期,将种子转接到发酵培养基中,添加玻璃珠打散菌体,同时添加辅酶促进转化,控制pH值、温度及碳源浓度,置于摇床上振荡发酵,发酵液经酶解使菌丝形成原生质体破壁单细胞,再经加热、脱色,过滤、超滤、反复醇沉、干燥沉淀,即可制得高纯度猴头菌多糖,该多糖纯度可达90%以上,含量约为5~6g/L。
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