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1. WO2020133989 - AMINO ACID DEHYDROGENASE MUTANT AND USE THEREOF IN SYNTHESIS OF L-GLUFOSINATE

Publication Number WO/2020/133989
Publication Date 02.07.2020
International Application No. PCT/CN2019/093453
International Filing Date 28.06.2019
IPC
C12N 9/06 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
02Oxidoreductases (1.), e.g. luciferase
06acting on nitrogen containing compounds as donors (1.4, 1.5, 1.7)
C12N 15/54 2006.1
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
52Genes encoding for enzymes or proenzymes
54Transferases (2)
C12N 1/21 2006.1
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
20Bacteria; Culture media therefor
21modified by introduction of foreign genetic material
C12P 13/04 2006.1
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
13Preparation of nitrogen-containing organic compounds
04Alpha- or beta-amino acids
CPC
C12N 9/0014
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes; Proenzymes; Compositions thereof
0004Oxidoreductases (1.)
0012acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
0014acting on the CH-NH2 group of donors (1.4)
C12N 9/0016
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes; Proenzymes; Compositions thereof
0004Oxidoreductases (1.)
0012acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
0014acting on the CH-NH2 group of donors (1.4)
0016with NAD or NADP as acceptor (1.4.1)
C12P 13/04
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
13Preparation of nitrogen-containing organic compounds
04Alpha- or beta- amino acids
C12Y 104/01015
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
YENZYMES
104Oxidoreductases acting on the CH-NH2 group of donors (1.4)
01with NAD+ or NADP+ as acceptor (1.4.1)
01015Lysine dehydrogenase (1.4.1.15)
Applicants
  • 浙江工业大学 ZHEJIANG UNIVERSITY OF TECHNOLOGY [CN]/[CN]
Inventors
  • 薛亚平 XUE, Yaping
  • 程峰 CHENG, Feng
  • 李恒 LI, Heng
  • 郑裕国 ZHENG, Yuguo
  • 徐建妙 XU, Jianmiao
Agents
  • 杭州天正专利事务所有限公司 TIANZHENG PATENT ATTORNEYS
Priority Data
201811621667.828.12.2018CN
Publication Language Chinese (ZH)
Filing Language Chinese (ZH)
Designated States
Title
(EN) AMINO ACID DEHYDROGENASE MUTANT AND USE THEREOF IN SYNTHESIS OF L-GLUFOSINATE
(FR) MUTANT DE DÉSHYDROGÉNASE D'ACIDE AMINÉ ET SON UTILISATION DANS LA PRÉPARATION DE L-GLUFOSINATE
(ZH) 一种氨基酸脱氢酶突变体及其在合成L-草铵膦中的应用
Abstract
(EN)
Disclosed are an amino acid dehydrogenase mutant and the use thereof in the synthesis of L-glufosinate. The amino acid dehydrogenase mutant is obtained by performing a single mutation or multiple mutations on amino acids at positions 95, 108, 172 and 303 shown in SEQ ID No.2. The specific enzyme activity of the prepared amino acid dehydrogenase mutant DyGDH-F95I-A108T-R172P -R303H is 33 times higher than that of the maternal aldo-keto reductase, and the maximum feeding amount of a substrate 2-carbonyl-4-(hydroxyl methyl phosphinyl)-butyric acid reaches 500 mM. The amino acid dehydrogenase mutant has better industrial application prospects. By using the amino acid dehydrogenase mutant to produce L-glufosinate, the reaction time is significantly shortened, from generally taking 20 hours to now only taking 120 minutes, showing that the amino acid dehydrogenase mutant has a good application prospect.
(FR)
L'invention concerne un mutant de déshydrogénase d'acide aminé et son utilisation dans la synthèse de L-glufosinate. Le mutant de déshydrogénase d'acide aminé est obtenu par réalisation d'une seule mutation ou de multiples mutations sur des acides aminés aux positions 95, 108, 172 et 303 représentées dans SEQ ID No.2 L'activité enzymatique spécifique du mutant de déshydrogénase d'acide aminé DyGDH-F95I-A108T-R172P-R303H préparé est de 33 fois supérieure à celle de l'aldo-céto réductase maternelle, et la quantité d'alimentation maximale d'un substrat d'acide 2-carbonyl-4-(hydroxyl méthyl phosphinyl)-butyrique s'élève à 500 mM. Le mutant de déshydrogénase d'acide aminé présente de meilleures perspectives d'application industrielles. En utilisant le mutant de déshydrogénase d'acide aminé pour produire du L-glufosinate, le temps de réaction est significativement raccourci, passant de généralement 20 heures habituellement à uniquement 120 minutes maintenant, montrant que le mutant de déshydrogénase d'acide aminé présente une bonne perspective d'application.
(ZH)
一种氨基酸脱氢酶突变体及其在合成L-草铵膦中的应用,所述氨基酸脱氢酶突变体是将SEQ ID No.2所示氨基酸第95位、108位、172位、303位进行单突变或多突变获得的。制备的氨基酸脱氢酶突变体DyGDH-F95I-A108T-R172P -R303H的比酶活较母本醛酮还原酶提升了33倍,最大底物2-羰基-4-(羟基甲基氧膦基)-丁酸投料达到500mM,该氨基酸脱氢酶突变体更具工业应用前景。利用该氨基酸脱氢酶突变体生产L-草铵膦,反应时间显著缩短,一般的过程需要20小时,而现在反应时间仅需要120分钟,这显示该氨基酸脱氢酶突变体具有很好的工业应用前景。
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