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1. WO2020133772 - METHOD AND SYSTEM FOR PREPARING RECOMBINANT ADENO-ASSOCIATED VIRUS, AND RECOMBINANT BACMID

Publication Number WO/2020/133772
Publication Date 02.07.2020
International Application No. PCT/CN2019/080295
International Filing Date 29.03.2019
IPC
C12N 15/866 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
85for animal cells
86Viral vectors
866Baculoviral vectors
C12N 7/01 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
7Viruses, e.g. bacteriophages; Compositions thereof; Preparation or purification thereof
01Viruses, e.g. bacteriophages, modified by introduction of foreign genetic material
CPC
C07K 14/005
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
005from viruses
C12N 15/86
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
85for animal cells
86Viral vectors
C12N 2710/14043
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2710dsDNA Viruses
00011dsDNA Viruses
14011Baculoviridae
14041Use of virus, viral particle or viral elements as a vector
14043viral genome or elements thereof as genetic vectore
C12N 2710/14122
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2710dsDNA Viruses
00011dsDNA Viruses
14011Baculoviridae
14111Nucleopolyhedrovirus, e.g. autographa californica nucleopolyhedrovirus
14122New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
C12N 2710/14144
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2710dsDNA Viruses
00011dsDNA Viruses
14011Baculoviridae
14111Nucleopolyhedrovirus, e.g. autographa californica nucleopolyhedrovirus
14141Use of virus, viral particle or viral elements as a vector
14144Chimeric viral vector comprising heterologous viral elements for production of another viral vector
C12N 2710/14151
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2710dsDNA Viruses
00011dsDNA Viruses
14011Baculoviridae
14111Nucleopolyhedrovirus, e.g. autographa californica nucleopolyhedrovirus
14151Methods of production or purification of viral material
Applicants
  • 中国科学院精密测量科学与技术创新研究院 INNOVATION ACADEMY FOR PRECISION MEASUREMENT SCIENCE AND TECHNOLOGY, CHINESE ACADEMY OF SCIENCES [CN]/[CN]
Inventors
  • 吴阳 WU, Yang
  • 徐富强 XU, Fuqiang
  • 梅婷 MEI, Ting
  • 蒋良玉 JIANG, Liangyu
  • 韩增鹏 HAN, Zengpeng
Agents
  • 武汉臻诚专利代理事务所(普通合伙) WUHAN GENUIRY INTELLECTUAL PROPERTY FIRM (GENERAL PARTNER)
Priority Data
201811618542.X28.12.2018CN
Publication Language Chinese (ZH)
Filing Language Chinese (ZH)
Designated States
Title
(EN) METHOD AND SYSTEM FOR PREPARING RECOMBINANT ADENO-ASSOCIATED VIRUS, AND RECOMBINANT BACMID
(FR) PROCÉDÉ ET SYSTÈME DE PRÉPARATION DE VIRUS ADÉNO-ASSOCIÉ RECOMBINÉ, ET BACMIDE RECOMBINÉ
(ZH) 重组腺相关病毒的制备方法、系统及重组杆粒
Abstract
(EN)
Provided are a method and a system for preparing a recombinant adeno-associated virus (rAAV), and a recombinant bacmid. The method comprises: (1) respectively preparing a shuttle plasmid and a corresponding recombinant bacmid containing baculovirus genome; (2) integrating a rAAV core expression element carrying a heterologous functional gene segment with other expression cassettes of functional protein components necessary for rAAV production to obtain a recombinant bacmid containing the recombinant baculovirus genomes that produce rAAV; and (3) transfecting the recombinant bacmid into a host cell line for cultivation. The system comprises a shuttle plasmid and a corresponding recombinant bacmid containing baculovirus genome. The recombinant bacmid comprises at least one expression cassette for a functional protein component required to produce rAAV.
(FR)
Procédé et système de préparation d'un virus adéno-associé recombiné (AAVr), et bacmide recombiné. Le procédé comprend les étapes suivantes : (1) préparation d'un plasmide navette et d'un bacmide recombiné correspondant contenant un génome de baculovirus; (2) intégration d'un élément d'expression de base AAVr portant un segment de gène fonctionnel hétérologue avec d'autres cassettes d'expression de composants protéiques fonctionnels nécessaires à la production de AAVr pour obtenir un bacmide recombiné contenant les génomes de baculovirus recombinés produisant un AAVr; et (3) transfection du bacmide recombiné dans une lignée cellulaire hôte pour la culture. Le système comprend un plasmide navette et un bacmide recombiné correspondant contenant un génome de baculovirus. Le bacmide recombiné comprend au moins une cassette d'expression pour un composant protéique fonctionnel nécessaire pour produire un AAVr.
(ZH)
提供了一种重组腺相关病毒(rAAV)的制备方法、系统及重组杆粒。方法包括:(1)分别制备穿梭质粒及其相应含有杆状病毒基因组的重组杆粒;(2)将带有异源功能性基因片段的rAAV核心表达元件与其他产生rAAV所必需的功能蛋白组分的表达框整合获得包含生产所述rAAV的重组杆状病毒基因组的重组杆粒;(3)转染到宿主细胞系培养。该系统包括穿梭质粒及其相应含有杆状病毒基因组的重组杆粒。重组杆粒至少包含一种产生rAAV所需的功能蛋白组分的表达框。
Also published as
KR1020217019980
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