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1. WO2020133713 - STRUCTURE AND APPLICATION OF DOUBLE-STRANDED OLIGONUCLEOTIDE NUCLEIC ACID PROBE

Publication Number WO/2020/133713
Publication Date 02.07.2020
International Application No. PCT/CN2019/077879
International Filing Date 12.03.2019
IPC
C12Q 1/686 2018.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
6844Nucleic acid amplification reactions
686Polymerase chain reaction
C12N 15/11 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
CPC
C12Q 1/686
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6844Nucleic acid amplification reactions
686Polymerase chain reaction [PCR]
Applicants
  • 中国人民解放军军事科学院军事医学研究院 ACADEMY OF MILITARY MEDICAL SCIENCES [CN]/[CN]
Inventors
  • 王升启 WANG, Shengqi
  • 刘琪琦 LIU, Qiqi
  • 刘丽艳 LIU, Liyan
  • 张影 ZHANG, Ying
  • 赵怡 ZHAO, Yi
Agents
  • 北京超凡志成知识产权代理事务所(普通合伙) CHOFN INTELLECTUAL PROPERTY
Priority Data
201811643407.029.12.2018CN
Publication Language Chinese (ZH)
Filing Language Chinese (ZH)
Designated States
Title
(EN) STRUCTURE AND APPLICATION OF DOUBLE-STRANDED OLIGONUCLEOTIDE NUCLEIC ACID PROBE
(FR) STRUCTURE ET APPLICATION D'UNE SONDE D'ACIDE NUCLÉIQUE OLIGONUCLÉOTIDIQUE DOUBLE BRIN
(ZH) 一种双链寡核苷酸核酸探针的结构和用途
Abstract
(EN)
Disclosed by the present application are a structure of a double-stranded oligonucleotide nucleic acid probe, a method of use and applications thereof in nucleic acid fluorescence qualitative and quantitative analysis, medical diagnosis and life science researches. The double-stranded oligonucleotide nucleic acid probe is composed of two completely or partially base-complementary oligonucleotide strands; the end of each oligonucleotide strand may be connected to a fluorescent group or a corresponding fluorescent quenching group; and the two oligonucleotide probe strands may hybridize with a target nucleic acid sequence to be tested.
(FR)
La présente invention concerne une structure d'une sonde d'acide nucléique d'oligonucléotide double brin, un procédé d'utilisation et des applications correspondantes dans une analyse qualitative et quantitative par fluorescence d'acide nucléique, un diagnostic médical et des recherches en sciences de la vie. La sonde d'acide nucléique oligonucléotidique à double brin est composée de deux brins d'oligonucléotide entièrement ou partiellement complémentaires; l'extrémité de chaque brin d'oligonucléotide pouvant être connectée à un groupe fluorescent ou à un groupe d'extinction fluorescent correspondant; et les deux brins de sonde d'oligonucléotide pouvant s'hybrider avec une séquence d'acide nucléique cible à tester.
(ZH)
本申请公开一种双链寡核苷酸核酸探针结构、使用方法及其在核酸荧光定性、定量分析、医学诊断和生命科学研究中的用途。双链寡核苷酸核酸探针是由两条完全或部分碱基互补的寡核苷酸链组成,每条寡核苷酸链的末端可以连接荧光基团或对应的荧光淬灭基团,两条寡核苷酸探针链均可与待测目的核酸序列杂交结合。
Also published as
CH10202100000759
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