Processing

Please wait...

Settings

Settings

Goto Application

1. WO2020118200 - METHODS FOR PREPARING CDNA SAMPLES FOR RNA SEQUENCING, AND CDNA SAMPLES AND USES THEREOF

Publication Number WO/2020/118200
Publication Date 11.06.2020
International Application No. PCT/US2019/064977
International Filing Date 06.12.2019
IPC
C12Q 1/686 2018.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
6844Nucleic acid amplification reactions
686Polymerase chain reaction
C12Q 1/6853 2018.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
6844Nucleic acid amplification reactions
6853using modified primers or templates
C12Q 1/6848 2018.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
6844Nucleic acid amplification reactions
6848characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
Applicants
  • QIAGEN SCIENCES, LLC [US]/[US]
Inventors
  • WANG, Yexun
  • LEWIS, Marcus
Agents
  • KIM, Judith
Priority Data
62/776,69707.12.2018US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) METHODS FOR PREPARING CDNA SAMPLES FOR RNA SEQUENCING, AND CDNA SAMPLES AND USES THEREOF
(FR) PROCÉDÉS DE PRÉPARATION D'ÉCHANTILLONS D'ADNC POUR SÉQUENÇAGE D'ARN, ET ÉCHANTILLONS D'ADNC ET LEURS UTILISATIONS
Abstract
(EN)
The invention relates to methods for preparing cDNA samples for RNA sequencing using random priming oligonucleotides comprising a cell barcode (cID), a unique molecular index (UMI), and a random sequence region, and performing a reverse transcription reaction (RT). The invention also relates to cDNA samples prepared by the methods and uses thereof.
(FR)
L'invention concerne des procédés de préparation d'échantillons d'ADNc pour le séquençage d'ARN à l'aide d'oligonucléotides à amorçage aléatoire comprenant un code-barres cellulaire (cID), un indice moléculaire unique (UMI), et une région de séquence aléatoire, et la mise en oeuvre d'une réaction de transcription inverse (RT). L’invention concerne en outre des échantillons d'ADNc produits par lesdits procédés et leurs utilisations.
Latest bibliographic data on file with the International Bureau