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1. WO2020118043 - METHODS FOR PRODUCING RECOMBINANT PROTEINS

Publication Number WO/2020/118043
Publication Date 11.06.2020
International Application No. PCT/US2019/064665
International Filing Date 05.12.2019
IPC
C12N 15/70 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
70Vectors or expression systems specially adapted for E. coli
CPC
C12N 15/70
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
70Vectors or expression systems specially adapted for E. coli
C12R 1/19
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
RPROCESSES USING MICROORGANISMS
1Processes using microorganisms
01using bacteria or actinomycetales
185Escherichia
19Escherichia coli
Applicants
  • COHERUS BIOSCIENCES, INC. [US]/[US]
Inventors
  • WILLIAMS, Robert Neal
Agents
  • REITER, Tiffany
  • LAVOIE, Teresa A.
Priority Data
62/776,88007.12.2018US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) METHODS FOR PRODUCING RECOMBINANT PROTEINS
(FR) PROCÉDÉS DE PRODUCTION DE PROTÉINES RECOMBINÉES
Abstract
(EN)
Provided herein are methods of producing a recombinant protein that include: providing a bacterium including a nucleic acid encoding a recombinant protein; and culturing the bacterium in a liquid culture medium including about 0.3 mM to about 300 mM Mg2+ under conditions sufficient for the production and release of the recombinant protein into the culture medium.
(FR)
L'invention concerne des procédés de production d'une protéine recombinée consistant à forunir une bactérie comprenant un acide nucléique codant pour une protéine recombinée; et à cultiver la bactérie dans un milieu de culture liquide comprenant environ 0,3 mM à environ 300 mM de Mg2+ dans des conditions suffisantes pour la production et la libération de la protéine recombinée dans le milieu de culture.
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