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1. WO2020117968 - POLYMERASES, COMPOSITIONS, AND METHODS OF USE

Publication Number WO/2020/117968
Publication Date 11.06.2020
International Application No. PCT/US2019/064524
International Filing Date 04.12.2019
IPC
C12N 9/12 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
10Transferases (2.)
12transferring phosphorus containing groups, e.g. kinases (2.7)
C12Q 1/6869 2018.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
6869Methods for sequencing
CPC
C12N 9/1252
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes; Proenzymes; Compositions thereof
10Transferases (2.)
12transferring phosphorus containing groups, e.g. kinases (2.7)
1241Nucleotidyltransferases (2.7.7)
1252DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
C12Q 1/6869
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6869Methods for sequencing
C12Y 207/07007
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
YENZYMES
207Transferases transferring phosphorus-containing groups (2.7)
07Nucleotidyltransferases (2.7.7)
07007DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
Applicants
  • ILLUMINA, INC. [US]/[US]
  • ILLUMINA CAMBRIDGE LIMITED [GB]/[GB]
  • ILLUMINA SINGAPORE PTE. LTD. [SG]/[SG]
Inventors
  • KLAUSING, Kay
  • GHOMI, Hamed Tabatabaei
  • GOLYNSKIY, Misha
  • NIRANTAR, Saurabh
  • MCDONALD, Seth
  • PEISAJOVICH, Sergio
Agents
  • PROVENCE, David
Priority Data
62/775,66205.12.2018US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) POLYMERASES, COMPOSITIONS, AND METHODS OF USE
(FR) POLYMÉRASES, COMPOSITIONS ET PROCÉDÉS D'UTILISATION
Abstract
(EN)
Presented herein are altered polymerase enzymes for improved incorporation of nucleotides and nucleotide analogues, in particular altered polymerases that maintain high fidelity under reduced incorporation times, as well as methods and kits using the same.
(FR)
L'invention concerne des enzymes de polymérase modifiées pour une incorporation améliorée de nucléotides et d'analogues de nucléotides, en particulier des polymérases modifiées qui conservent une haute fidélité dans des temps d'incorporation réduits, ainsi que des procédés et des kits les utilisant.
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