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1. WO2020117849 - METHODS OF ANALYSIS USING IN-SAMPLE CALIBRATION CURVE BY MULTIPLE ISOTOPOLOGUE REACTION MONITORING

Publication Number WO/2020/117849
Publication Date 11.06.2020
International Application No. PCT/US2019/064299
International Filing Date 03.12.2019
IPC
G01N 33/68 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68involving proteins, peptides or amino acids
CPC
G01N 2458/15
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
2458Labels used in chemical analysis of biological material
15Non-radioactive isotope labels, e.g. for detection by mass spectrometry
G01N 33/6848
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68involving proteins, peptides or amino acids
6803General methods of protein analysis not limited to specific proteins or families of proteins
6848Methods of protein analysis involving mass spectrometry
Applicants
  • BRISTOL-MYERS SQUIBB COMPANY [US]/[US]
Inventors
  • GU, Huidong
  • ZHAO, Yue
  • ZENG, Jianing
Agents
  • KIM, Ji Eun
  • BANOWIT, Donald R.
  • BEMBEN, Richard M.
  • BEZOS, Salvador M.
  • BLOCK, Daniel S.
  • BODENSTEIN, Matthew S.
  • CALVO, Paul A.
  • CAMARCE, Christian A,
  • CIMBALA, Michele A.
  • COLLER III, Richard D.
  • CONKLIN, Kyle E.
  • CORNWELL, David K.S.
  • COVERT, John M.
  • DURKIN, Tracy-Gene G.
  • EISENBERG, Jason D.
  • ELLISON, Eldora L.
  • ESMOND, Robert W.
  • FEATHERSTONE, Donald J.
  • FITZSIMMONS, Jason A.
  • FRUEAUF, Jeremiah B.
  • GAJEWSKI, Daniel
  • GILLENTINE, Marsha Rose
  • GOLDSTEIN, Jorge A.
  • HELVEY, Jeffrey T.
  • HICKS, Ross
  • HOLOUBEK, Michelle K.
  • JACKMAN, Peter A.
  • KENTON, JR., Lestin L.
  • LEE, Michael Q.
  • LONGSWORTH, Gaby L.
  • MILLONIG, Robert C.
  • MUTSCHELKNAUS, Joseph
  • NANNENGA-COMBS, Bonnie
  • POWERS III, R. Wilson
  • RAY, Michael B.
  • RICHARDSON, Ryan C.
  • RYGIEL, Mark W.
  • SHEA JR., Timothy J.
  • SOKOHL, Robert E.
  • SPECHT, Michael D.
  • STEFFE, Eric K.
  • STERLING, Deborah A.
  • STERNE, Robert Greene
Priority Data
62/775,31804.12.2018US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) METHODS OF ANALYSIS USING IN-SAMPLE CALIBRATION CURVE BY MULTIPLE ISOTOPOLOGUE REACTION MONITORING
(FR) PROCÉDÉS D'ANALYSE UTILISANT UNE COURBE D'ÉTALONNAGE DANS UN ÉCHANTILLON PAR SURVEILLANCE DE RÉACTION D'ISOTOPOLOGUES MULTIPLES
Abstract
(EN)
This disclosure provides several methods in LC-MS/MS analysis: (1) a method of LC-MS/MS analysis technique to determine the analyte concentration of a sample wherein an In-Sample Calibration Curve (ISCC) is used instead of an external calibration curve through monitoring of multiple isotopologue transitions of an added stable isotopically labeled (SIL) analyte in each sample via MS/MS in multiple isotopologue reaction monitoring (MIRM) mode; (2) a method of LC-MS/MS analysis to determine the analyte concentration of a sample wherein a One-Sample Multipoint External Calibration Curve (OSMECC) is used instead of a multisample external calibration curve; and (3) a method of LC-MS/MS analysis to determine the analyte concentration of a sample with an analyte concentration beyond the assay's ULOQ wherein isotope sample dilution is used instead of diluting sample physically during sample preparation based on calculating the isotopic abundance of the MIRM channel monitored.
(FR)
La présente invention concerne plusieurs procédés d'analyse par CL-SM/SM : (1) un procédé de technique d'analyse CL-SM/SM pour déterminer la concentration d'analyte d'un échantillon, une courbe d'étalonnage dans l'échantillon (ISCC) étant utilisée au lieu d'une courbe d'étalonnage externe par surveillance de transitions d'isotopologues multiples d'un analyte marqué isotopiquement stable (SIL) ajouté dans chaque échantillon par SM/SM en mode surveillance de réaction d'isotopologues multiples (MIRM) ; (2) un procédé d'analyse CL-SM/SM pour déterminer la concentration d'analyte d'un échantillon, une courbe d'étalonnage externe à points multiples d'échantillon unique (OSMECC) étant utilisée au lieu d'une courbe d'étalonnage externe multi-échantillons ; et (3) un procédé d'analyse LC-MS/MS pour déterminer la concentration d'analyte d'un échantillon avec une concentration d'analyte supérieure à la LSDQ du dosage, la dilution d'échantillon d'isotopes étant utilisée au lieu de diluer l'échantillon physiquement pendant la préparation d'échantillon sur la base du calcul de l'abondance isotopique du canal MIRM surveillé.
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