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1. WO2020117553 - GENE SILENCING VIA GENOME EDITING

Publication Number WO/2020/117553
Publication Date 11.06.2020
International Application No. PCT/US2019/063200
International Filing Date 26.11.2019
IPC
A01H 1/04 2006.01
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
HNEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
1Processes for modifying genotypes
04Processes of selection
A01H 5/10 2018.01
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
HNEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
5Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
10Seeds
C07K 14/415 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
415from plants
C12N 15/10 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
C12N 15/29 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
29Genes encoding plant proteins, e.g. thaumatin
C12N 15/82 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
82for plant cells
CPC
C12N 15/82
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
82for plant cells ; , e.g. plant artificial chromosomes (PACs)
C12N 15/8213
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
82for plant cells ; , e.g. plant artificial chromosomes (PACs)
8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
8213Targeted insertion of genes into the plant genome by homologous recombination
C12N 15/8241
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
82for plant cells ; , e.g. plant artificial chromosomes (PACs)
8241Phenotypically and genetically modified plants via recombinant DNA technology
Applicants
  • SYNGENTA CROP PROTECTION AG [CH]/[CH]
  • SYNGENTA BIOTECHNOLOGY CHINA CO., LTD. [CN]/[CN] (CN)
Inventors
  • LYU, Jian
  • CHEN, Xi
  • YU, Kun
  • LIANG, Dawei
  • ZHOU, Hongju
  • XU, Jianping
Common Representative
  • SYNGENTA CROP PROTECTION AG
Priority Data
PCT/CN2018/11915504.12.2018CN
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) GENE SILENCING VIA GENOME EDITING
(FR) SILENÇAGE GÉNIQUE PAR LE BIAIS D'UNE ÉDITION GÉNOMIQUE
Abstract
(EN)
The present invention relates to methods and compositions for gene silencing by genome editing. In some embodiments, nucleases are provided selected from the group consisting of meganucleases (MNs), zinc-finger nucleases (ZFNs), transcription-activator like effector nucleases (TALENs), Cas9 nuclease, Cfp1 nuclease, dCas9-FokI, dCpf1-FokI, chimeric Cas9/Cpf1-cytidine deaminase, chimeric Cas9/Cpf1-adenine deaminase, chimeric FEN1-FokI, and Mega-TALs, a nickase Cas9 (nCas9), chimeric dCas9 non-FokI nuclease and dCpf1 non-FokI nuclease. Additionally, the present invention relates to methods and compositions for gene silencing by genome editing. Also provided are methods and compositions for rearranging a chromosome by genome editing.
(FR)
La présente invention se rapporte à des procédés et à des compositions pour un silençage génique au moyen d'une édition génomique. Dans certains modes de réalisation, la présente invention porte sur des nucléases sélectionnées dans le groupe constitué par des méganucléases (MN pour MegaNuclease), des nucléases à doigts de zinc (ZFN pour Zinc-Finger Nuclease), des nucléases effectrices de type activateur de transcription (TALEN pour Transcription-Activator Like Effector Nuclease), une nucléase Cas9, une nucléase Cfp1, une dCas9-FokI, une dCpf1-FokI, la Cas9/Cpf1-cytidine désaminase chimérique, la Cas9/Cpf1-adénine désaminase chimérique, la FEN1-FokI chimérique et les méga-TAL, une Cas9 nickase (nCas9), une nucléase non FokI dCas9 chimérique et une nucléase non FokI dCpf1. De plus, la présente invention se rapporte à des procédés et à des compositions pour un silençage génique au moyen d'une édition génomique. La présente invention porte également sur des procédés et sur des compositions pour réagencer un chromosome au moyen d'une édition génomique.
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