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1. WO2020116955 - NUCLEIC ACID EXTRACTION METHOD FOR DIRECT ANALYTIC DIAGNOSIS OF CARBAPENEMASE-PRODUCING GENES FROM STOOL OR RECTAL SWAB SPECIMEN

Publication Number WO/2020/116955
Publication Date 11.06.2020
International Application No. PCT/KR2019/017083
International Filing Date 05.12.2019
IPC
C12Q 1/6806 2018.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction assay
C12N 15/10 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
CPC
C12N 15/10
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
C12Q 1/6806
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
Applicants
  • 가톨릭대학교 산학협력단 THE CATHOLIC UNIVERSITY OF KOREA INDUSTRY-ACADEMIC COOPERATION FOUNDATION [KR]/[KR]
Inventors
  • 박연준 PARK, Yeon Joon
  • 이지은 LEE, Ji Eun
Agents
  • 엄명용 EOM, Myung Yong
Priority Data
10-2018-015491605.12.2018KR
Publication Language Korean (KO)
Filing Language Korean (KO)
Designated States
Title
(EN) NUCLEIC ACID EXTRACTION METHOD FOR DIRECT ANALYTIC DIAGNOSIS OF CARBAPENEMASE-PRODUCING GENES FROM STOOL OR RECTAL SWAB SPECIMEN
(FR) PROCÉDÉ D'EXTRACTION D'ACIDE NUCLÉIQUE POUR LE DIAGNOSTIC ANALYTIQUE DIRECT DE GÈNES PRODUISANT DES CARBAPÉNÈMASES À PARTIR D'UN PRÉLÈVEMENT DE SELLES OU D'ÉCOUVILLON RECTAL
(KO) 대변 또는 직장 면봉 검체에서 직접 카바페넴아제 생성 유전자의 분석적 진단을 위한 핵산 추출 방법
Abstract
(EN)
The present invention is for extracting a nucleic acid for directly detecting carbapenemase-producing genes from a stool or rectal swab specimen. The present invention has excellent extractability compared to a nucleic acid extraction method by means of an existing standard scheme, has great economical advantage in terms of time and cost, and allows reduced manpower or cost from using an expensive instrument, and thus the nucleic acid extraction efficiency can be enhanced.
(FR)
La présente invention concerne l'extraction d'un acide nucléique pour la détection directe de gènes produisant des carbapénèmases à partir d'un prélèvement de selles ou d'écouvillon rectal. La présente invention présente une excellente extractabilité par rapport à un procédé d'extraction d'acide nucléique au moyen d'un schéma standard existant, elle présente un avantage économique en termes de temps et de coût, et elle permet de réduire la main d'oeuvre ou le coût induit par l'utilisation d'un instrument onéreux, et ainsi l'efficacité d'extraction d'acide nucléique peut être améliorée.
(KO)
본 발명은, 대변 또는 rectal swab 검체에서 직접 카바페넴아제 생성 유전자의 검출을 위해 핵산을 추출하기 위한 것으로, 기존의 표준법에 의한 핵산 추출방법보다 추출 능력이 우수함은 물론, 시간 빛 비용면에서 매우 경제적인 장점과, 고가의 장비 사용에 따른 인력이나 비용을 줄일 수 있어서 핵산 추출의 효율성을 향상시킬 수 있는 장점을 기대할 수 있게 한다.
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