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1. WO2020116330 - GLUCOSE DEHYDROGENASE

Publication Number WO/2020/116330
Publication Date 11.06.2020
International Application No. PCT/JP2019/046686
International Filing Date 29.11.2019
IPC
C12N 5/10 2006.01
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5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
10Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
C12N 1/15 2006.01
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1Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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1Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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16Yeasts; Culture media therefor
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C12N 1/21 2006.01
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1Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
20Bacteria; Culture media therefor
21modified by introduction of foreign genetic material
C12N 15/53 2006.01
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15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
52Genes encoding for enzymes or proenzymes
53Oxidoreductases (1)
C12Q 1/26 2006.01
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1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
26involving oxidoreductase
Applicants
  • 天野エンザイム株式会社 AMANO ENZYME INC. [JP]/[JP]
Inventors
  • 杉浦 敏行 SUGIURA Toshiyuki
  • 井戸 宏樹 IDO Hiroki
Agents
  • 萩野 幹治 HAGINO Mikiharu
Priority Data
2018-22858305.12.2018JP
Publication Language Japanese (JA)
Filing Language Japanese (JA)
Designated States
Title
(EN) GLUCOSE DEHYDROGENASE
(FR) GLUCOSE DÉSHYDROGÉNASE
(JA) グルコースデヒドロゲナーゼ
Abstract
(EN)
The present invention addresses the problem of providing FAD-GDH having high thermal stability and, in particular, having improved practical applicability as a glucose sensor. Provided is a glucose dehydrogenase having: (a) an amino acid sequence which shows at least 90% amino acid sequence identity with SEQ ID NO: 1 or SEQ ID NO: 13, and in which the amino acid corresponding to amino acid at position 429 of the amino acid sequence SEQ ID NO: 1 is phenylalanine (F); or (b) an amino acid sequence which shows at least 90% amino acid sequence identity with SEQ ID NO: 2 or SEQ ID NO: 14, and in which the amino acid corresponding to amino acid at position 429 of the amino acid sequence SEQ ID NO: 2 is phenylalanine (F). The relative remaining activity after a 10 minute treatment at 50°C is at least 50%.
(FR)
La présente invention permet de fournir une FAD-GDH ayant une stabilité thermique élevée et, en particulier, ayant une applicabilité pratique améliorée en tant que capteur de glucose. L'invention concerne une glucose déshydrogénase ayant : (A) une séquence d'acides aminés présentant au moins 90% d'identité de séquence d'acides aminés avec SEQ ID NO : 1 ou SEQ ID NO : 13, et dans laquelle l'acide aminé correspondant à l'acide aminé en position 429 de la séquence d'acides aminés SEQ ID NO : 1 est la phénylalanine (F); ou (b) une séquence d'acides aminés présentant au moins 90 % d'identité de séquence d'acides aminés avec SEQ ID NO : 2 et/ou SEQ ID NO: 14, et dans laquelle l'acide aminé correspondant à l'acide aminé en position 429 de la séquence d'acides aminés SEQ ID NO : 2 Est la phénylalanine (F). L'activité restante relative après un traitement de 10 minutes à 50 °C est d'au moins 50 %.
(JA)
熱安定性が高く、特にグルコースセンサ用としての実用性が向上したFAD-GDHを提供することを課題とする。(a)配列番号1又は配列番号13のアミノ酸配列に90%以上の同一性を示し、且つ配列番号1のアミノ酸配列の429位アミノ酸に相当するアミノ酸はフェニルアラニン(F)であるアミノ酸配列、又は(b)配列番号2又は配列番号14のアミノ酸配列に90%以上の同一性を示し、且つ配列番号2のアミノ酸配列の429位アミノ酸に相当するアミノ酸はフェニルアラニン(F)であるアミノ酸配列を有し、50℃で10分処理後の相対残存活性が50%以上である、グルコースデヒドロゲナーゼが提供される。
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