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1. WO2020115760 - COMPOSITIONS, METHODS, SYSTEMS TO ADDRESS STRESS, STARVATION, IMPROVED HEALTH, IMPROVED WATER QUALITY, PRODUCTIVITY AND LIFE EXPECTANCY OF FISHES

Note: Text based on automatic Optical Character Recognition processes. Please use the PDF version for legal matters

[ EN ]

AMENDED CLAIMS

received by the International Bureau on 13 May 2020 (13.05.2020)

1. A composition for aquaculture characterised by:

addressing the stress and starvation of edible and ornamental fishes throughout the distribution chain which includes farms, aquariums, transportation bags, aquatic environments, at retail outlets and for end users,

enabling close to 0% mortalities of fishes throughout the distribution chains; at farms, at aquariums, during transportations (Death On Arrival - DOA, Death After Arrival - DAA, Death After 7 Days Of Arrival - DA7), retail outlets and end users,

pre-treatment of fishes to achieve close to 0% mortalities of fishes throughout the distribution chains; at farms, at aquariums, during transportations (Death On Arrival -DOA, Death After Arrival - DAA, Death After 7 Days Of Arrival - DA7), retailers and end users,

serving as a probiotic, enabling better health, digestion, immune system, disease control, promotor of reproductions, improvement in color.

serving as a prebiotic,

serving to improve water quality by reducing high levels of nitrates, nitrites and ammonia close to 0 ppm and maintaining at that level,

making fishes more compatible and less aggressive,

usage of water lesser than the currently prescribed amount and replacing water with lesser frequency,

enabling immediate acclimatization to the new environment the fishes are introduced, using probiotic bacteria along with nutritive media, culture media as component(s) of the composition,

variations in the composition and their proportions in permutations and combinations and along corresponding systems and methods renders the various benefits characteristic of the composition, and

systems to achieve close to 0 ppm of nitrates, nitrites and ammonia at aquariums and Fish Only Tanks, Systems to incorporate the composition into biofiltration system of Recirculatory Aquaculture Systems (RAS), Systems to enable the production unit of product to be an integral setup of farms, Systems to use the composition for edible fishes; this system is used as a precautionary measure as these fishes are used for human consumption.

2. The composition of Claim 1, comprising a probiotic bacteria,

3. The composition of Claim 1, comprising bacteria with at least one of Accession Numbers MCC0148, MCC0149, and MCC0150.

4. The composition of Claim 3, wherein the bacteria have sequence similarity with Pseudomonas species in the range of 75% to 85%.

5. The composition of Claim 3, wherein the bacteria include bacteria with only one of Accession Numbers MCC0148, MCC0149, and MCC0150.

6. The composition of Claim 3, wherein the bacteria include bacteria from more than one of Accession Numbers MCC0148, MCC0149, and MCC0150.

7. The composition of Claim 3, wherein the bacteria are selected from Accession Numbers MCC0148, MCC0149, and MCC0150 to be used in combinations having a ratio in the range of (0.5:0.5: 1) to (1 : 1 :3).

8. The composition of Claim 1, comprising culture media which are chemically defined.

9. The composition(s) of Claim 1, wherein the culture media is customised in its formulation(s).

10. The composition of Claim 1, comprising nutritive media which are chemically defined and serve as a prebiotic.

11. The composition of Claim 1, comprising nutritive media which serves as a prebiotic.

12. The composition of one of Claim 8 and Claim 9, wherein the culture media also serve as a nutritive media.

13. The composition of one of Claim 8 and Claim 9, wherein the culture media is proportional to the amount to be used for a given volume, as given in instructions by the manufacturer of the media.

14. The composition of one of Claim 8 and Claim 9, wherein the culture media is in a dehydrated form or a suspension.

15. The composition of one of Claim 10 and Claim 11, wherein the nutritive media is proportional to the amount to be used for a given volume, as given in instructions by the manufacturer of the media.

16. The composition of one of Claim 10 and Claim 11 wherein the nutritive media is in a dehydrated form or a suspension.

17. The composition of Claim 13, wherein the amount of culture media is selected during preparation depending on at least one of where the composition is to be used, bacterial proportions and density, fish taxonomy, water quality, and fish density.

18. The composition of Claim 15 wherein the amount of nutritive media is selected during preparation depending on at least one of where the composition is to be used, bacterial proportions and density, fish taxonomy, water quality, and fish density.

19. The composition of one of Claim 2 and Claim 3 wherein the bacteria are incubated for different time points.

20. The composition of Claim 7, wherein the bacteria are incubated for a minimum duration of 40-48 hours.

21. The composition of one of Claim 2 and Claim 3 wherein bacteria are used either individually or in combinations, in the composition(s), in various proportions.

22. The composition of Claim 7, wherein the composition(s) with bacterial proportions of (1 :2:2) - (MCC0148 : MCC0149 : MCC0150) incubated for a minimum of 48 hours are used for Koi fishes, for the various characteristics mentioned in Claim 1.

23. The composition of Claim 7, wherein the composition(s) with bacterial proportions of (0.77:0.8:2) for (MCC0148 : MCC0149 : MCC0150) incubated for a minimum of 48 hours are used for Koi fishes, for the various characteristics mentioned in Claim 1.

24. A method using the composition of Claim 1, comprising:

placing Koi fishes in the presence of the composition for at least nine days, wherein: the Koi fishes are thereby capable of maintaining their blood lactate levels at no more than 0.2 mmol/L at the end of said nine days while the Koi fishes are subjected to stressful conditions, such as placing two Koi fishes in a bowl containing about 900 ml of water with only aeration facility, aerations stopped for hours, water not changed for said nine days, and fish feed given twice a day and few pellets.

25. The composition of Claim 1 , wherein the composition(s) are fine tuned for a specific fish type while maintaining the characteristics of Claim 1.

26. The composition of Claim 1, wherein the composition is capable of being used for all Edible fishes and Ornamental fishes.

27. The composition of Claim 1, wherein the parameters for bacterial growth which could include incubation time, container used, preference to culture media, in shaker, cell pellets being used or suspension culture of the bacteria are altered based on the specifications to be met while preparing the composition.

28. The composition of Claim 1, wherein the composition is highly specific for a fish type or fish taxonomy.

29. The composition of Claim 1 , wherein the composition has variations based on the kind of culture media chosen, the nutritive component chosen, the proportions of the bacteria used in the composition(s); in permutations or combinations or if composition(s) are prepared using the individual bacteria, the proportion(s) of the various components of the composition(s), whether the bacteria are used as suspension culture or as cell pellet, the extent of role of the composition(s) in improving water quality, serving as probiotic and prebiotic taking into account the water quality to be addressed, the density of fishes, fish type, their food feed patterns.

30. A method using the composition of Claim 1, comprising:

preparing the composition(s) based on whether the composition is to be used at farms or aquariums, or during transportation, warehouses or at retail outlets, or for end users, the fish type, the quality of water, density of fishes, fish feed patterns.

31. A method for preparing the composition of Claim 1 , comprising:

obtaining aliquots of the bacterial suspension incubated for a minimum of 48 hours, adding the culture media, nutritive media (the culture media could also serve as nutritive media), and

mixing for about 1- 5 minutes, such that the resulting composition is prepared for introduction into tanks, farms or transportation bags, or warehouses.

32. The method of Claim 31, further comprising introducing the resulting composition into said tanks, farms or transportation bags, or warehouses using a volume of approximately 1 ml of the composition in 1 litre of water.

33. The method of Claim 31, wherein the method is the simplest method or process to prepare the composition.

34. The method of Claim 31, wherein the method could have variations in terms of the form in which the bacteria is being used in the composition(s) - either as cell mass or suspension, the incubation times chosen for the bacteria, the proportions of culture media added, the kind of culture media being used; defined or customised, the nutritive media which could be defined or customised, the combination of bacteria being used in the composition(s) or if one bacterial type being used, the density of fishes, the amount of water, quality of water, fish density, fish feed patterns in deciding the proportion(s) and component(s) of the composition(s).

35. A system using the composition of Claim 1, wherein the system is adapted for ensuring bacterial cells do not get in direct contact with the edible fishes, as a precautionary measure.

36. A system using the composition of Claim 1, wherein the system is adapted for reducing and maintaining nitrates, nitrites and ammonia levels close to 0 ppm as the composition is used with fishes.

37. The system of Claim 36, comprising rocks, such as lava rocks, to which the composition is introduced to reduce and maintain close to 0 ppm of nitrates, nitrites and ammonia.

38. The system of Claim 36, comprising a spherical container with perforations into which rocks, such as lava rocks, could be densely packed, wherein the spherical container is adapted to receive the composition.

39. The system of Claim 36, wherein the system is integrated into Recirculatory Aquaculture Systems (RAS) for reducing and maintaining nitrates, nitrites and ammonia levels close to 0 ppm.

40. A system using the composition of Claim 1 , wherein the system is custom designed or uses existing technologies to integrate a production unit using the composition at a farm, an aquarium or a warehouse.