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1. WO2020115286 - CELL-PERMEABLE FLUOROGENIC FLUOROPHORES

Publication Number WO/2020/115286
Publication Date 11.06.2020
International Application No. PCT/EP2019/084006
International Filing Date 06.12.2019
IPC
C09B 57/02 2006.01
CCHEMISTRY; METALLURGY
09DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
BORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES; MORDANTS; LAKES
57Other synthetic dyes of known constitution
02Coumarine dyes
C09B 11/02 2006.01
CCHEMISTRY; METALLURGY
09DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
BORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES; MORDANTS; LAKES
11Diaryl- or triarylmethane dyes
02derived from diarylmethanes
C09B 11/24 2006.01
CCHEMISTRY; METALLURGY
09DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
BORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES; MORDANTS; LAKES
11Diaryl- or triarylmethane dyes
04derived from triarylmethanes
10Amino derivatives of triarylmethanes
24Phthaleins containing amino groups
G01N 33/533 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
531Production of immunochemical test materials
532Production of labelled immunochemicals
533with fluorescent label
G01N 1/30 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
1Sampling; Preparing specimens for investigation
28Preparing specimens for investigation
30Staining; Impregnating
CPC
C09B 11/02
CCHEMISTRY; METALLURGY
09DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
BORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES ; , e.g. PIGMENTS; MORDANTS; LAKES
11Diaryl- or thriarylmethane dyes
02derived from diarylmethanes
C09B 11/24
CCHEMISTRY; METALLURGY
09DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
BORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES ; , e.g. PIGMENTS; MORDANTS; LAKES
11Diaryl- or thriarylmethane dyes
04derived from triarylmethanes ; , i.e. central C-atom is substituted by amino, cyano, alkyl
10Amino derivatives of triarylmethanes
24Phthaleins containing amino groups ; ; Phthalanes; Fluoranes; Phthalides; Rhodamine dyes; Phthaleins having heterocyclic aryl rings; Lactone or lactame forms of triarylmethane dyes
C09B 57/02
CCHEMISTRY; METALLURGY
09DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
BORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES ; , e.g. PIGMENTS; MORDANTS; LAKES
57Other synthetic dyes of known constitution
02Coumarine dyes
G01N 1/30
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
1Sampling; Preparing specimens for investigation
28Preparing specimens for investigation ; including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
30Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
G01N 33/533
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
531Production of immunochemical test materials
532Production of labelled immunochemicals
533with fluorescent label
Applicants
  • MAX-PLANCK-GESELLSCHAFT ZUR FÖRDERUNG DER WISSENSCHAFTEN E. V. [DE]/[DE]
Inventors
  • JOHNSSON, Kai
  • WANG, Lu
Agents
  • JUNGHANS, Claas
Priority Data
18210676.506.12.2018EP
19180481.417.06.2019EP
19181362.519.06.2019EP
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) CELL-PERMEABLE FLUOROGENIC FLUOROPHORES
(FR) FLUOROPHORES FLUOROGÉNIQUES À PERMÉABILITÉ CELLULAIRE
Abstract
(EN)
The present invention relates to rhodamine-type fluorophores being present in two states, a cell-permeable non-florescent form and a fluorescent form. The present invention also relates to use of the fluorophores in staining and live cell fluorescence imaging. The compounds have general formula (10) or the general formula (10'):
(FR)
La présente invention concerne des fluorophores de type rhodamine présents dans deux états, une forme non fluorescente à perméabilité cellulaire et une forme fluorescente. La présente invention concerne également l'utilisation de ces fluorophores pour la coloration et l'imagerie par fluorescence de cellules vivantes.
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