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1. WO2020113365 - GENETICALLY ENGINEERED BACTERIUM PRODUCING HIGH YIELD OF LIPOPEPTIDES AND USE THEREOF

Publication Number WO/2020/113365
Publication Date 11.06.2020
International Application No. PCT/CN2018/118893
International Filing Date 03.12.2018
IPC
C12N 1/21 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
20Bacteria; Culture media therefor
21modified by introduction of foreign genetic material
C12P 21/00 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
21Preparation of peptides or proteins
C12R 1/125 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C-C12Q75
1Microorganisms
01Bacteria or actinomycetales
07Bacillus
125Bacillus subtilis
C12R 1/085 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C-C12Q75
1Microorganisms
01Bacteria or actinomycetales
07Bacillus
085Bacillus cereus
C12R 1/38 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C-C12Q75
1Microorganisms
01Bacteria or actinomycetales
38Pseudomonas
CPC
C12P 21/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
21Preparation of peptides or proteins
C12R 1/085
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
RPROCESSES USING MICROORGANISMS
1Processes using microorganisms
01using bacteria or actinomycetales
07Bacillus
085Bacillus cereus
C12R 1/125
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
RPROCESSES USING MICROORGANISMS
1Processes using microorganisms
01using bacteria or actinomycetales
07Bacillus
125Bacillus subtilis
C12R 1/38
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
RPROCESSES USING MICROORGANISMS
1Processes using microorganisms
01using bacteria or actinomycetales
38Pseudomonas
Applicants
  • 清华大学 TSINGHUA UNIVERSITY [CN]/[CN]
Inventors
  • 于慧敏 YU, Huimin
  • 王苗苗 WANG, Miaomiao
Agents
  • 北京众合诚成知识产权代理有限公司 BEIJING ZHCC INTELLECTUAL PROPERTY CO., LTD
Priority Data
Publication Language Chinese (ZH)
Filing Language Chinese (ZH)
Designated States
Title
(EN) GENETICALLY ENGINEERED BACTERIUM PRODUCING HIGH YIELD OF LIPOPEPTIDES AND USE THEREOF
(FR) BACTÉRIE GÉNÉTIQUEMENT MODIFIÉE PRODUISANT UN RENDEMENT ÉLEVÉ DE LIPOPEPTIDES ET SON UTILISATION
(ZH) 一种高产脂肽的基因工程菌及其应用
Abstract
(EN)
A genetically engineered bacterium producing a high yield of lipopeptides and use thereof. The genetically engineered bacterium producing a high yield of lipopeptides is constructed by inactivating at least one gene in the fourth stage and the fifth stage of spore synthesis in an original strain, and can maintain complete cell morphology in a high concentration of a surfactin. Compared with the starting strain, no spore is produced during fermentation, and lipopeptide production is significantly increased. The highest surfactin production in a shake flask reaches 9.9 g/L, which is 25% higher than that of the starting strain, and the cell activity is also enhanced.
(FR)
Bactérie génétiquement modifiée produisant un haut rendement de lipopeptides et son utilisation. La bactérie génétiquement modifiée produisant un rendement élevé de lipopeptides est construite par inactivation d'au moins un gène dans la quatrième étape et la cinquième étape de synthèse de spores dans une souche originelle, et peut maintenir une morphologie cellulaire complète dans une concentration élevée d'une surfactine. Par rapport à la souche de départ, aucune spore n'est produite pendant la fermentation, et la production de lipopeptides est significativement augmentée. La production de surfactine la plus élevée dans un ballon d'agitation atteint 9,9 g/L, qui est 25 % plus haut que celui de la souche de départ, et l'activité cellulaire est également améliorée.
(ZH)
一种高产脂肽的基因工程菌及其应用。所述高产脂肽的基因工程菌,其是使原始菌株中芽孢合成第四阶段和第五阶段中的至少一个基因失活构建而成,其在高浓度表面活性素环境下能够保持完整的细胞形态;与出发菌株相比,发酵过程中无芽孢产生且显著提高脂肽产量,摇瓶中表面活性素产量最高达到9.9g/L,比出发菌株提高了25%,细胞活性也得到增强。
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