Processing

Please wait...

Settings

Settings

Goto Application

1. WO2020113173 - MULTIOMIC ANALYSIS OF CELL ANALYTES USING MICROFLUIDIC SYSTEMS

Publication Number WO/2020/113173
Publication Date 04.06.2020
International Application No. PCT/US2019/063866
International Filing Date 29.11.2019
IPC
B01L 3/00 2006.01
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
3Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
C12M 1/00 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1Apparatus for enzymology or microbiology
C12N 1/00 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
CPC
B01L 2200/0668
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
2200Solutions for specific problems relating to chemical or physical laboratory apparatus
06Fluid handling related problems
0647Handling flowable solids, e.g. microscopic beads, cells, particles
0668Trapping microscopic beads
B01L 2300/0816
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
2300Additional constructional details
08Geometry, shape and general structure
0809rectangular shaped
0816Cards, e.g. flat sample carriers usually with flow in two horizontal directions
B01L 2400/086
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
2400Moving or stopping fluids
08Regulating or influencing the flow resistance
084Passive control of flow resistance
086using baffles or other fixed flow obstructions
B01L 3/502761
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
3Containers or dishes for laboratory use, e.g. laboratory glassware
50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
502with fluid transport, e.g. in multi-compartment structures
5027by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
502761specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
C12M 1/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
1Apparatus for enzymology or microbiology
C12N 1/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1Microorganisms, e.g. protozoa; Compositions thereof
Applicants
  • CORNELL UNIVERSITY [US]/[US]
Inventors
  • CRAIGHEAD, Harold G.
  • TIAN, Harvey C.
  • LIN, David M.
  • BISOGNI, Adam J.
Agents
  • GONSALVES, Andrew K.
Priority Data
62/772,62128.11.2018US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) MULTIOMIC ANALYSIS OF CELL ANALYTES USING MICROFLUIDIC SYSTEMS
(FR) ANALYSE MULTI-OMIQUE D'ANALYTES CELLULAIRES À L'AIDE DE SYSTÈMES MICROFLUIDIQUES
Abstract
(EN)
A method for isolating one or more distinct analyte component from a cell sample is disclosed, as well as processes for testing and analyzing the distinct analyte components. The distinct analyte components include: (i) a total protein fraction; (ii) a plasma membrane protein fraction; (iii) a total RNA fraction; (iv) a cytosolic RNA fraction; (v) a cytosolic protein fraction; (vi) a nuclear RNA fraction; (vii) a nuclear protein fraction; (viii) a chromatin fraction comprising genomic DNA (gDNA); (ix) a gDNA markers fraction. This method involves the use of microfluidic device having a cell capture component and a nucleic acid entanglement component, where the cell capture component includes a cell capture array having a plurality of cell capture micropillars, where the nucleic acid entanglement component includes a nucleic acid entanglement array having a plurality of nucleic acid entanglement micropillars, and where the microfluidic device operates under continuous flow conditions.
(FR)
L'invention concerne un procédé pour isoler un ou plusieurs constituants d'analyte distincts d'un échantillon cellulaire, ainsi que des processus de test et d'analyse des constituants d'analyte distincts. Les constituants d'analyte distincts comprennent : (i) une fraction de protéine totale; (ii) une fraction de protéine de membrane de plasma; (iii) une fraction d'ARN totale; (iv) une fraction d'ARN cytosolique; (v) une fraction de protéine cytosolique; (vi) une fraction d'ARN nucléaire; (vii) une fraction de protéine nucléaire; (viii) une fraction de chromatine comprenant de l'ADN génomique (ADNg); (ix) une fraction de marqueurs d'ADNg. Ce procédé fait appel à l'utilisation d'un dispositif microfluidique comportant un composant de capture de cellule et un composant d'enchevêtrement d'acide nucléique, le composant de capture de cellule comprenant un réseau de capture de cellule comportant une pluralité de micropiliers de capture de cellule, le composant d'enchevêtrement d'acide nucléique comprenant un réseau d'enchevêtrement d'acide nucléique comportant une pluralité de micropiliers d'enchevêtrement d'acide nucléique, et le dispositif microfluidique fonctionnant dans des conditions de flux continu.
Latest bibliographic data on file with the International Bureau