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1. WO2020112964 - SEQUENCING BY COALASCENCE

Publication Number WO/2020/112964
Publication Date 04.06.2020
International Application No. PCT/US2019/063551
International Filing Date 27.11.2019
IPC
C12Q 1/6876 2018.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
C12Q 1/6874 2018.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
6869Methods for sequencing
6874involving nucleic acid arrays, e.g. sequencing by hybridisation
G16B 30/20 2019.01
GPHYSICS
16INFORMATION AND COMMUNICATION TECHNOLOGY SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
30ICT specially adapted for sequence analysis involving nucleotides or amino acids
20Sequence assembly
CPC
C12Q 1/6874
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6869Methods for sequencing
6874involving nucleic acid arrays, e.g. sequencing by hybridisation
C12Q 1/6876
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
G16B 25/20
GPHYSICS
16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
25ICT specially adapted for hybridisation; ICT specially adapted for gene or protein expression
20Polymerase chain reaction [PCR]; Primer or probe design; Probe optimisation
G16B 30/20
GPHYSICS
16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
30ICT specially adapted for sequence analysis involving nucleotides or amino acids
20Sequence assembly
G16B 40/10
GPHYSICS
16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
40ICT specially adapted for biostatistics; ICT specially adapted for bioinformatics-related machine learning or data mining, e.g. knowledge discovery or pattern finding
10Signal processing, e.g. from mass spectrometry [MS] or from PCR
Applicants
  • XGENOMES CORP. [US]/[US]
Inventors
  • MIR, Kalim
Agents
  • LOVEJOY, Brett, A.
  • KWAK, Byung, Cheol
  • NG, Jennifer
  • TALUKDER, Gargi
  • MANN, Jeffry, S.
Priority Data
62/772,97929.11.2018US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) SEQUENCING BY COALASCENCE
(FR) SÉQUENCAGE PAR COALESCENCE
Abstract
(EN)
A method of sequencing a single, elongated target polynucleotide molecule can include the steps of seeding a plurality of separately resolvable origins of polynucleotide synthesis along the single, elongated target polynucleotide; contacting the target polynucleotide with a polymerase and labelled nucleotides; incorporating a labelled nucleotide, using the polymerase, into a plurality of sequence fragments complementary to the target polynucleotide and originating from the origins of polynucleotide synthesis; identifying and storing the identity and positions of the labelled nucleotide incorporated into each of the plurality of sequence fragments; and repeating the incorporating and identifying steps until adjacent sequence fragments coalesce and result in continuous sequence reads spanning two or more adjacent sequence fragments.
(FR)
Un procédé de séquençage d'une seule molécule polynucléotidique cible allongée peut consister à ensemencer une pluralité d'origines de synthèse de polynucléotides pouvant être résolues séparément, le long du polynucléotide cible allongé unique; à mettre en contact le polynucléotide cible avec une polymérase et des nucléotides marqués; à incorporer un nucléotide marqué, à l'aide de la polymérase, en une pluralité de fragments de séquence complémentaires du polynucléotide cible et provenant des origines de synthèse de polynucléotides; à identifier et stocker l'identité et les positions du nucléotide marqué incorporé dans chaque fragment de la pluralité de fragments de séquence; et à répéter les étapes d'incorporation et d'identification jusqu'à ce que des fragments de séquence adjacents coalescent et conduisent à des lectures de séquence continue couvrant au moins deux fragments de séquence adjacents.
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