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1. WO2020112742 - ALKALINE PURIFICATION OF SPIDER SILK PROTEINS

Publication Number WO/2020/112742
Publication Date 04.06.2020
International Application No. PCT/US2019/063208
International Filing Date 26.11.2019
IPC
B33Y 70/00 2020.01
BPERFORMING OPERATIONS; TRANSPORTING
33ADDITIVE MANUFACTURING TECHNOLOGY
YADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
70Materials specially adapted for additive manufacturing
C07K 14/435 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435from animals; from humans
C12N 5/00 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
CPC
C07K 14/435
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435from animals; from humans
C12N 5/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor;
Applicants
  • BOLT THREADS, INC. [US]/[US]
Inventors
  • MUI, Phillip
  • MUTALIK, Ritu, Bansal
  • LI, Simon
  • CHAN, Scott
Agents
  • WIENS, Mayim
  • ARGENTIERI, Steven, R.
  • ASHRAF, Shovon
  • BRODOWSKI, Michael
  • BUCHMAN, Joshua, J.
Priority Data
62/772,58828.11.2018US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) ALKALINE PURIFICATION OF SPIDER SILK PROTEINS
(FR) PURIFICATION ALCALINE DE PROTÉINES DE SOIE D'ARAIGNÉE
Abstract
(EN)
The present disclosure relates to methods of producing and purifying synthetic block copolymer proteins, expression constructs for their secretion, recombinant microorganisms for their production, and synthetic fibers comprising these proteins that recapitulate many properties of natural silk.
(FR)
La présente invention concerne des procédés et des compositions visant à la production et la purification de protéines copolymères séquencés synthétiques, des constructions d'expression pour leur sécrétion, des microorganismes de recombinaison pour leur production et des fibres synthétiques comprenant ces protéines qui cumulent de nombreuses propriétés de la soie naturelle.
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