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1. WO2020112723 - SYSTEMS AND METHODS FOR FACILITATING CLONE SELECTION

Publication Number WO/2020/112723
Publication Date 04.06.2020
International Application No. PCT/US2019/063177
International Filing Date 26.11.2019
IPC
G06K 9/00 2006.01
GPHYSICS
06COMPUTING; CALCULATING OR COUNTING
KRECOGNITION OF DATA; PRESENTATION OF DATA; RECORD CARRIERS; HANDLING RECORD CARRIERS
9Methods or arrangements for reading or recognising printed or written characters or for recognising patterns, e.g. fingerprints
G06K 9/62 2006.01
GPHYSICS
06COMPUTING; CALCULATING OR COUNTING
KRECOGNITION OF DATA; PRESENTATION OF DATA; RECORD CARRIERS; HANDLING RECORD CARRIERS
9Methods or arrangements for reading or recognising printed or written characters or for recognising patterns, e.g. fingerprints
62Methods or arrangements for recognition using electronic means
CPC
G06K 9/0014
GPHYSICS
06COMPUTING; CALCULATING; COUNTING
KRECOGNITION OF DATA; PRESENTATION OF DATA; RECORD CARRIERS; HANDLING RECORD CARRIERS
9Methods or arrangements for reading or recognising printed or written characters or for recognising patterns, e.g. fingerprints
00127Acquiring and recognising microscopic objects, e.g. biological cells and cellular parts
0014Pre-processing, e.g. image segmentation
G06K 9/00147
GPHYSICS
06COMPUTING; CALCULATING; COUNTING
KRECOGNITION OF DATA; PRESENTATION OF DATA; RECORD CARRIERS; HANDLING RECORD CARRIERS
9Methods or arrangements for reading or recognising printed or written characters or for recognising patterns, e.g. fingerprints
00127Acquiring and recognising microscopic objects, e.g. biological cells and cellular parts
00147Matching; Classification
G06K 9/627
GPHYSICS
06COMPUTING; CALCULATING; COUNTING
KRECOGNITION OF DATA; PRESENTATION OF DATA; RECORD CARRIERS; HANDLING RECORD CARRIERS
9Methods or arrangements for reading or recognising printed or written characters or for recognising patterns, e.g. fingerprints
62Methods or arrangements for recognition using electronic means
6267Classification techniques
6268relating to the classification paradigm, e.g. parametric or non-parametric approaches
627based on distances between the pattern to be recognised and training or reference patterns
Applicants
  • AMGEN INC. [US]/[US]
Inventors
  • YUAN, Yu
  • MILNE, Graham, F.
  • WANG, Tony, Y.
  • LE, Kim, H.
  • TAN, Glenn
Agents
  • BATEMAN, Andrew, W.
Priority Data
62/774,15430.11.2018US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) SYSTEMS AND METHODS FOR FACILITATING CLONE SELECTION
(FR) SYSTÈMES ET PROCÉDÉS POUR FACILITER LA SÉLECTION DE CLONES
Abstract
(EN)
A method for facilitating clone selection includes generating time-sequence images of a well containing a medium, including a first image and a later, second image. The method also includes detecting, by one or more processors analyzing the first image, one or more candidate objects depicted in the first image, and, for each of the candidate objects, determining whether the object is a single cell by analyzing an image of the object using a convolutional neural network. The method further includes detecting, by analyzing the second image with the processor(s), a cell colony depicted in the second image, and determining, by the processor(s), whether the colony was formed from only one cell based at least on whether each candidate object was determined to be a single cell. The method further includes generating, by the processor(s), output data indicating whether the colony was formed from only one cell.
(FR)
L'invention concerne un procédé pour faciliter la sélection de clones qui consiste à générer des images en séquence temporelle d'un puits contenant un milieu, comprenant une première image et une seconde image ultérieure. Le procédé consiste également à détecter, par un ou plusieurs processeurs analysant la première image, un ou plusieurs objets candidats représentés dans la première image, et, pour chaque objet candidat, déterminer si l'objet est une cellule unique par analyse d'une image de l'objet à l'aide d'un réseau neuronal convolutif. Le procédé consiste en outre à détecter, par analyse de la seconde image avec le ou les processeurs, une colonie de cellules représentée dans la seconde image, et déterminer, par le ou les processeurs, si la colonie a été formée à partir d'une seule cellule au moins sur la base du fait que chaque objet candidat a été déterminé comme étant une cellule unique. Le procédé consiste en outre à générer, par le ou les processeurs, des données de sortie indiquant si la colonie a été formée à partir d'une seule cellule.
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