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1. WO2020111958 - A DEVICE FOR CONDUCTING BIOLOGICAL AMPLIFICATION REACTIONS

Note: Text based on automatic Optical Character Recognition processes. Please use the PDF version for legal matters

[ EN ]

Claims

1. Device for conducting amplification reaction of biological samples with a system for independent control of the temperature of test tubes in a heating assembly comprising a multipart heating slot located in the cooling system housing, a control system, and a power supply system, characterised in that the heating assembly comprises at least one heating slot (100) comprising a metal heating sleeve (101) wound around with a bifilar winding wire made of enamelled winding wire (102), which is covered with a composite polymer layer (103), wherein a temperature sensor (104) is located on the surface of the winding wire, and at least one heating slot is mounted on the PCB control board

(105) located on the cooling system housing (112).

2. The device according to claims 1 to 2, characterised in that the cooling system housing (112) comprises at least one fan (117) connected at the outlet to a least one housing (112) of the air duct (114) for placing the PCB board (105) with at least one heating slot (100).

3. The device according to claims 1 to 2, characterised in that the housing (112) comprises two side walls (120) connected with each other by means of a third wall (115, 121).

4. The device according to claims 1 to 3, characterised in that the temperature ramp of the heating sleeve is at least 20°C/s.

5. The device according to claims 1 to 4, characterised in that the temperature sensor (104) is a PtlOO or PtlOOO sensor.

6. The device according to claims 1 to 5, characterised in that the PCB board (105) is connected to the control system and to the power supply.

7. The device according to claims 1 to 6, characterised in that the cross-section of the heating slot

(100) is circular.

8. The device according to claims 1 to 7 characterised in that the longitudinal cross-section of the heating slot (100) has a tapered cone shape.

9. The device according to claims 1 to 8, characterised in that the thickness of the heating sleeve

(101) does not exceed 0.1 mm.

10. The device according to claims 1 to 9, characterised in that the heating sleeve (101) is made of copper.

11. The device according to claims 1 to 10, characterised in that the winding wire (102) is made of copper.

12. The device according to claims 1 to 11, characterised in that the winding wire (102) is enamelled with a polyurethane based enamel.

13. The device according to claims 1 to 12, characterised in that the PCB board (105) comprises a slit

(106) reducing the heat transfer to the PCB board (105) from the heating slot (100).

14. The device according to claims 1 to 13, characterised in that an inner chamber (118) comprising the excitation and fluorescence measurement arrangement (108, 109, 110, 111) is located in the housing (112) of the air duct (114).

15. The device according to claims 1 to 14, characterised in that the excitation and fluorescence measurement arrangement is mounted on the housing (112) of the air duct (114) and comprises: an optic fibre (108) of the fluorescence excitation diode connected to the diode (109) for fluorescence excitation, an optic fibre (110) with a filter for the fluorescence detector connected to the fluorescence detector (111), wherein the excitation and fluorescence measurement arrangement is separated from the air duct (114) with an insulating surface (115), through which optic fibres of the excitation (108) and fluorescence measurement (110) arrangement are introduced.

16. The device according to claims 1 to 15, characterised in that the excitation and fluorescence measurement arrangement is connected to the heating slot by means of optic fibres (108, 110).