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1. WO2020111678 - METHOD FOR EXTRACTING EXTRACELLULAR MATRIX USING SUPERCRITICAL FLUID, AND EXTRACELLULAR MATRIX BIOMATERIAL FOR TISSUE REGENERATION PRODUCED THEREBY

Publication Number WO/2020/111678
Publication Date 04.06.2020
International Application No. PCT/KR2019/016189
International Filing Date 22.11.2019
IPC
A61L 27/36 2006.01
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION, OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICAL ARTICLES
27Materials for prostheses or for coating prostheses
36containing ingredients of undetermined constitution or reaction products thereof
C12N 5/077 2010.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07Animal cells or tissues
071Vertebrate cells or tissues, e.g. human cells or tissues
077Mesenchymal cells, e.g. bone cells, cartilage cells, marrow stromal cells, fat cells or muscle cells
C12N 5/00 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
CPC
A61L 27/3633
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION, OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICAL ARTICLES
27Materials for ; grafts or; prostheses or for coating ; grafts or; prostheses
36containing ingredients of undetermined constitution or reaction products thereof ; , e.g. transplant tissue, natural bone, extracellular matrix
3604characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
3633Extracellular matrix [ECM]
A61L 27/3683
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION, OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICAL ARTICLES
27Materials for ; grafts or; prostheses or for coating ; grafts or; prostheses
36containing ingredients of undetermined constitution or reaction products thereof ; , e.g. transplant tissue, natural bone, extracellular matrix
3683subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment
A61L 27/3691
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION, OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICAL ARTICLES
27Materials for ; grafts or; prostheses or for coating ; grafts or; prostheses
36containing ingredients of undetermined constitution or reaction products thereof ; , e.g. transplant tissue, natural bone, extracellular matrix
3683subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment
3691characterised by physical conditions of the treatment, e.g. applying a compressive force to the composition, pressure cycles, ultrasonic/sonication or microwave treatment, lyophilisation
C12N 5/0081
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor;
0081Purging biological preparations of unwanted cells
C12N 5/0653
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor;
06Animal cells or tissues; Human cells or tissues
0602Vertebrate cells
0652Cells of skeletal and connective tissues; Mesenchyme
0653Adipocytes; Adipose tissue
Applicants
  • 주식회사 도프 DOF INC. [KR]/[KR]
Inventors
  • 신용우 SHIN, Yong-Woo
  • 김규병 KIM, Kyu-Byung
  • 노성래 NO, Seong-Rae
  • 설윤희 SEOL, Yoon-Hee
  • 신지훈 SHIN, Jee-Hoon
  • 김형순 KIM, Hyoung-Soon
Agents
  • 특허법인(유한) 대아 DAE-A INTELLECTUAL PROPERTY CONSULTING
Priority Data
10-2018-015005728.11.2018KR
Publication Language Korean (KO)
Filing Language Korean (KO)
Designated States
Title
(EN) METHOD FOR EXTRACTING EXTRACELLULAR MATRIX USING SUPERCRITICAL FLUID, AND EXTRACELLULAR MATRIX BIOMATERIAL FOR TISSUE REGENERATION PRODUCED THEREBY
(FR) PROCÉDÉ D’EXTRACTION DE MATRICE EXTRACELLULAIRE METTANT EN ŒUVRE UN LIQUIDE SUPERCRITIQUE, ET BIOMATÉRIAU À MATRICE EXTRACELLULAIRE POUR LA RÉGÉNÉRATION TISSULAIRE AINSI PRODUIT
(KO) 초임계유체를 이용한 세포외기질 추출방법 및 이에 따라 제조된 조직 재생용 세포외기질 생체소재
Abstract
(EN)
The present invention provides a method for extracting an extracellular matrix using a supercritical fluid, the method comprising: (a) a step for injecting extracted adipose tissue into a reactor; (b) a step for preparing a supercritical fluid by pressurizing a solvent; and (c) a step for introducing the supercritical fluid into the reactor to remove cells from the adipose tissue, and degreasing to extract an extracellular matrix.
(FR)
La présente invention fournit un procédé d’extraction d’une matrice extracellulaire à l'aide d'un liquide supercritique, le procédé comprenant : (a) une étape d’injection du tissu adipeux extrait dans un réacteur ; (b) une étape de préparation d’un liquide supercritique par mise sous pression d’un solvant ; et (c) une étape d’introduction du liquide supercritique dans le réacteur pour retirer les cellules du tissu adipeux, et le dégraissage d’un extrait d’une matrice extracellulaire.
(KO)
본 발명은 (a) 추출한 지방조직을 반응기에 주입하는 단계; (b) 용매를 가압하여 초임계유체를 제조하는 단계; 및 (c) 상기 초임계유체를 상기 반응기에 투입하여 상기 지방조직에서 세포를 제거하고, 탈지하여 세포외기질을 추출하는 단계;를 포함하는 초임계유체를 이용한 세포외기질 추출방법을 제공한다.
Also published as
Latest bibliographic data on file with the International Bureau