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1. WO2020111372 - SERUM-FREE CRYOPRESERVATIVE SOLUTION FOR CRYOPRESERVATION OF HEPATOCYTES AND ENCAPSULATED HEPATOCYTE SPHEROIDS AND CRYOPRESERVATION METHOD USING SAME

Publication Number WO/2020/111372
Publication Date 04.06.2020
International Application No. PCT/KR2018/016677
International Filing Date 26.12.2018
IPC
A01N 1/02 2006.01
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
1Preservation of bodies of humans or animals, or parts thereof
02Preservation of living parts
C12N 5/071 2010.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07Animal cells or tissues
071Vertebrate cells or tissues, e.g. human cells or tissues
C12N 11/10 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
11Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
02Enzymes or microbial cells immobilised on or in an organic carrier
10the carrier being a carbohydrate
CPC
A01N 1/02
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES
1Preservation of bodies of humans or animals, or parts thereof
02Preservation of living parts
C12N 11/10
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
11Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
02Enzymes or microbial cells immobilised on or in an organic carrier
10the carrier being a carbohydrate
Applicants
  • 사회복지법인 삼성생명공익재단 SAMSUNG LIFE PUBLIC WELFARE FOUNDATION [KR]/[KR]
  • 라이프리버 주식회사 LIFELIVER CO., LTD. [KR]/[KR]
Inventors
  • 이지현 LEE, Ji Hyun
  • 이석구 LEE, Suk Koo
  • 이상훈 LEE, Sang Hoon
  • 이두훈 LEE, Doo Hoon
  • 노정권 NOH, Jeong Kwon
  • 정종갑 JUNG, Jong Gab
  • 윤희훈 YOON, Hee Hoon
Agents
  • 특허법인 다래 DARAE IP FIRM
Priority Data
10-2018-015107329.11.2018KR
Publication Language Korean (KO)
Filing Language Korean (KO)
Designated States
Title
(EN) SERUM-FREE CRYOPRESERVATIVE SOLUTION FOR CRYOPRESERVATION OF HEPATOCYTES AND ENCAPSULATED HEPATOCYTE SPHEROIDS AND CRYOPRESERVATION METHOD USING SAME
(FR) SOLUTION DE CRYOCONSERVATION SANS SÉRUM POUR LA CRYOCONSERVATION D'HÉPATOCYTES ET DE SPHÉROÏDES D'HÉPATOCYTES ENCAPSULÉS ET PROCÉDÉ DE CRYOCONSERVATION L'UTILISANT
(KO) 간세포 및 캡슐화한 간세포 구상체의 동결 보존을 위한 동결보존용 무혈청 용액 및 이를 이용한 동결보존법
Abstract
(EN)
The present invention relates to a serum-free solution for cryopreserving hepatocytes comprising a histidine-tryptophan-ketoglutaric acid (HTK) solution, dimethyl sulfoxide (DMSO), dexamethasone, and human serum albumin. By adding dexamethasone and/or N-acetylcysteine to conventional cryopreservative solutions, a hepatocyte survival rate, an ammonia removal rate, a urea synthesis rate, and an albumin secretion rate are enhanced as compared to conventional cryopreservative solutions.
(FR)
La présente invention concerne une solution sans sérum pour la cryoconservation d'hépatocytes comprenant une solution d'histidine-tryptophane-acide cétoglutarique (HTK), du diméthylsulfoxyde (DMSO), de la dexaméthasone et de la sérum albumine humaine. Par l'ajout de dexaméthasone et/ou de N-acétylcystéine à des solutions de cryopréservation classiques, un taux de survie des hépatocytes, un taux d'élimination d'ammoniac, un taux de synthèse d'urée et un taux de sécrétion d'albumine sont améliorés par comparaison avec les solutions de cryopréservation classiques.
(KO)
본 발명은 HTK(히스티딘-트립토판-케토글루타르산) 용액, 디메틸 설폭사이드(DMSO), 덱사메타손 및 인간 혈청 알부민을 포함하는 것을 특징으로 하는 무혈청 간세포 동결보존용액에 대한 것으로, 종래의 동결보존용액에 덱사메타손 및/또는 N-아세틸시스테인을 추가하여 종래의 동결보전용액보다 간세포의 생존율, 암모니아 제거율, 우레아 합성율 및 알부민 분비율이 향상된 것을 특징으로 한다.
Also published as
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