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1. WO2020111135 - METHOD FOR ANALYZING IMMUNE CELLS AND CELL ANALYSIS INSTRUMENT

Publication Number WO/2020/111135
Publication Date 04.06.2020
International Application No. PCT/JP2019/046410
International Filing Date 27.11.2019
IPC
G01N 33/49 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
483Physical analysis of biological material
487of liquid biological material
49blood
G01N 33/53 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
CPC
G01N 33/49
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
483Physical analysis of biological material
487of liquid biological material
49Blood
G01N 33/53
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
Applicants
  • シスメックス株式会社 SYSMEX CORPORATION [JP]/[JP]
Inventors
  • 岡 祐馬 OKA, Yuma
  • 柳田 匡俊 YANAGIDA, Masatoshi
  • 三輪 桂子 MIWA, Keiko
Agents
  • 野河 信太郎 NOGAWA, Shintaro
  • 甲斐 伸二 KAI, Shinji
  • 金子 裕輔 KANEKO, Yusuke
  • 稲本 潔 INAMOTO, Kiyoshi
Priority Data
2018-22365729.11.2018JP
Publication Language Japanese (JA)
Filing Language Japanese (JA)
Designated States
Title
(EN) METHOD FOR ANALYZING IMMUNE CELLS AND CELL ANALYSIS INSTRUMENT
(FR) PROCÉDÉ D'ANALYSE DE CELLULES IMMUNITAIRES ET INSTRUMENT D'ANALYSE CELLULAIRE
(JA) 免疫細胞の分析方法及び細胞分析装置
Abstract
(EN)
The present invention relates to a method for analyzing immune cells, the method including: a step for mixing whole blood containing immune cells and an immune stimulation factor in vitro, and labeling a target molecule of the immune cells; and a step for detecting the label to obtain information regarding the location of the target molecule on immune cells. The present invention also relates to an instrument that analyzes cells obtained by mixing whole blood containing immune cells and an immune stimulation factor in vitro, and causing a target molecule to be present on the immune cells.
(FR)
La présente invention concerne un procédé d'analyse de cellules immunitaires, le procédé comprenant : une étape consistant à mélanger le sang total contenant des cellules immunitaires et un facteur de stimulation immunitaire in vitro, et à marquer une molécule cible des cellules immunitaires; et une étape consistant à détecter le marqueur pour obtenir des informations concernant l'emplacement de la molécule cible sur des cellules immunitaires. La présente invention concerne également un instrument qui analyse des cellules obtenues par mélange du sang total contenant des cellules immunitaires et d'un facteur de stimulation immunitaire in vitro, et provoquant la présence d'une molécule cible sur les cellules immunitaires.
(JA)
本発明は、免疫細胞を含む全血と免疫刺激因子とをインビトロで混合し、免疫細胞の標的分子を標識する工程と、標識を検出することにより、免疫細胞における標的分子の局在に関する情報を取得する工程とを含む、免疫細胞の分析方法に関する。また、本発明は、免疫細胞を含む全血と免疫刺激因子とをインビトロで混合し、免疫細胞において標的分子の局在を生成することにより得られた細胞を分析する装置に関する。
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