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1. WO2020110083 - MATRIX-MEDIATED CELL CULTURE SYSTEM

Publication Number WO/2020/110083
Publication Date 04.06.2020
International Application No. PCT/IB2019/060330
International Filing Date 29.11.2019
IPC
A01G 33/00 2006.01
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
33Cultivation of seaweed
C12N 1/00 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
CPC
A01G 33/00
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
33Cultivation of seaweed ; or algae
C12N 1/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1Microorganisms, e.g. protozoa; Compositions thereof
C12N 11/14
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
11Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
14Enzymes or microbial cells immobilised on or in an inorganic carrier
Applicants
  • UNIVERSITY OF CAPE TOWN [ZA]/[ZA]
Inventors
  • STEVENS, Dylan Troy
  • RYBICKI, Edward Peter
  • HARRISON, Susan T L
Agents
  • SPOOR & FISHER
  • MOUBRAY, Hugh Robert
  • GILSON, David Grant
  • KEMP, Mark
  • WHITTAKER, Jonathan Denis
  • COCHRANE, David Hylton
  • ABRAMSON, Lance
  • MCKNIGHT, John Crawford
  • MAHOMED, Shanaaz
  • KAHN, Craig
  • BIAGIO, Dina
  • CILLIERS, Lodewyk Petrus
  • GRANT, Tyron James
  • BEHARIE, Tertia
  • VAN SCHALKWYK, Herman
  • PIENAAR, Danie
  • HANEKOM, Dirk Christiaan
Priority Data
2018/0807629.11.2018ZA
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) MATRIX-MEDIATED CELL CULTURE SYSTEM
(FR) SYSTÈME DE CULTURE CELLULAIRE INDUITE PAR MATRICE
Abstract
(EN)
The invention relates to a matrix-mediated algal cell culture system comprising a porous matrix, a microalgal cell culture comprising cells immobilised on the porous matrix, and a vector including a nucleic acid sequence encoding a heterologous polypeptide of interest, wherein immobilisation of microalgal cells on the porous matrix results in the formation of interstitial spaces between the microalgal cells to allow for increased contact of the microalgal cells with the vector compared with a culture of microalgal cells which are not immobilised on a porous matrix, thereby allowing for more efficient transfection of the microalgal cells with the vector. The invention also relates to methods of screening single species of microalgae and mixed ecology samples for the ability to be transfected using the algal cell culture system, and to methods for the production of heterologous polypeptides using the matrix-mediated cell culture system.
(FR)
L'invention concerne un système de culture de cellules algales induite par matrice comprenant une matrice poreuse, une culture de cellules microalgales comprenant des cellules immobilisées sur la matrice poreuse, et un vecteur contenant une séquence d'acides nucléiques codant pour un polypeptide hétérologue d'intérêt, l'immobilisation des cellules microalgales sur la matrice poreuse conduisant à la formation d'espaces interstitiels entre les cellules microalgales pour permettre un contact accru des cellules microalgales avec le vecteur par rapport à une culture de cellules microalgales qui ne sont pas immobilisées sur une matrice poreuse, ce qui permet une transfection plus efficace des cellules microalgales avec le vecteur. L'invention concerne également des procédés de sélection d'espèces uniques de microalgues et d'échantillons d'écologie mixte pour leur capacité de transfection grâce au système de culture de cellules algales, et des procédés de production de polypeptides hétérologues grâce au système de culture cellulaire induite par matrice.
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