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1. WO2020109631 - LAMININ IMMOBILIZATION, METHODS AND USES THEREOF

Publication Number WO/2020/109631
Publication Date 04.06.2020
International Application No. PCT/EP2019/083366
International Filing Date 02.12.2019
IPC
G01N 33/543 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
543with an insoluble carrier for immobilising immunochemicals
G01N 33/545 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
543with an insoluble carrier for immobilising immunochemicals
544the carrier being organic
545Synthetic resin
CPC
G01N 33/54353
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
543with an insoluble carrier for immobilising immunochemicals
54353with ligand attached to the carrier via a chemical coupling agent
G01N 33/545
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
543with an insoluble carrier for immobilising immunochemicals
544the carrier being organic
545Synthetic resin
Applicants
  • INEB - INSTITUTO NACIONAL DE ENGENHARIA BIOMÉDICA [PT]/[PT]
Inventors
  • GOMES MOREIRA PÊGO, Ana Paula
  • SANTANA RAMOS DE FREITAS AMARAL, Isabel Maria
  • DOS SANTOS BARROS, Daniela Filipa
Agents
  • PATENTREE
Priority Data
11518430.11.2018PT
11562804.07.2019PT
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) LAMININ IMMOBILIZATION, METHODS AND USES THEREOF
(FR) IMMOBILISATION DE LA LAMININE, PROCÉDÉS ET UTILISATIONS DE CELLE-CI
Abstract
(EN)
Laminin immobilization into diverse biological and synthetic matrices has been explored to replicate the microenvironment of stem cell niches and gain insight into the role of extracellular matrix (ECM) on stem cell behaviour. However, the site-specific immobilization of this heterotrimeric glycoprotein and, consequently, control over its orientation and bioactivity has been a challenge that has limited many of the explored strategies to date. An affinity-based approach was established that takes advantage of the native high affinity interaction between laminin and the human N-terminal agrin (hNtA) domain.
(FR)
Selon l'invention, l'immobilisation de la laminine dans diverses matrices biologiques et synthétiques a été explorée pour reproduire le micro-environnement de niches de cellules souches et mieux comprendre le rôle de la matrice extracellulaire (ECM) sur le comportement des cellules souches. Cependant, l'immobilisation spécifique au site de cette glycoprotéine hétérotrimérique et, par conséquent, le contrôle de son orientation et de sa bioactivité a été un défi qui a limité de nombreuses stratégies parmi celles explorées jusqu'à présent. Une approche basée sur l'affinité a été établie, qui tire avantage de l'interaction native de haute affinité entre la laminine et le domaine de l'agrine N-terminal humaine (hNtA).
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