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1. WO2020109507 - OSTEOBLASTS DERIVED FROM ORAL NEUROECTODERMAL STEM CELLS AND THEIR USE IN JAW REPAIR

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CLAIMS

1 ) A method for inducing differentiation of neuroectodemic oral stem cells into osteoblasts, said method comprising:

(a) a step of cell proliferation of neuroectodermal oral stem cells in a serum-free base culture medium supplemented with platelet lysate (PL) and human growth hormone (GH);

b) an osteoblastic differentiation step of the cell culture resulting from step a) in a serum-free base culture medium supplemented with a platelet lysate (PL) and at least one osteoblastic differentiation factor.

2) Method according to claim 1 , wherein the culture of step a) is carried out up to a confluence of 80%.

3) Method according to claim 1 or 2, wherein the culture of step b) is carried out during 21 to 28 days.

4) Method according to any of claim 1 to 3, wherein the culture medium of steps a) and b) is further supplemented with heparin.

5) Method according to claim 4, wherein the culture medium of step a) contains from 1 -2 lU/ml of heparin, and/or the culture medium of step b) contains from 0.1 -0.6 lU/ml of heparin.

6) Method according to any of claims 1 to 5, wherein said at least one osteoblastic differentiation factor is chosen from the group consisting of corticosteroids and glycerol phosphoric esters.

7) Method according to claim 6, wherein the said at least one differentiation factor is dexamethasone and/or b-glycerophosphate.

8) Method according to any of claims 1 to 7, wherein the neuroectoderm ic oral stem cells are derived from gingival tissue.

9) Cell population comprising osteoblasts produced by the method according to any of claims 1 to 8, said osteoblasts expressing the homeogen Msx2.

10) Cell population as defined in claim 9, for use in cell therapy.

11 ) Cell population for use according to claim 10, for the restoration of bone defects of maxillary bones.

12) Culture medium for the proliferation of oral neuroectodermal stem cells comprising a serum-free base culture medium supplemented with platelet lysate (PL) and human growth hormone (GH), and optionally heparin at a concentration ranging from 1 -2 lU/ml.

13) Culture medium for the differentiation of neuroectodermal oral stem cells into osteoblasts comprising a serum-free base culture medium supplemented with a platelet lysate (PL) and at least one osteoblastic differentiation factor, and optionally heparin at a concentration ranging from 0.1 -0.6 lU/ml.

14) Culture medium according to claim 13, wherein said at least one osteoblastic differentiation factor is chosen from the group consisting of corticosteroides and glycerol phosphoric esters.

15) Culture medium according to claim 14, wherein said at least one osteoblastic differentiation factor is dexamethasone and/or b-glycerophosphate.