Processing

Please wait...

Settings

Settings

Goto Application

1. WO2020109388 - WELLS FOR OPTIMIZED SAMPLE LOADING IN MICROFLUIDIC CHIPS

Note: Text based on automatic Optical Character Recognition processes. Please use the PDF version for legal matters

[ EN ]
CLAIMS

1. A loading well (320) configured to receive a drop of sample (313),

wherein the loading well (320) is an open cavity (324) comprising a wall (321) and a loading opening (325),

wherein the wall (321) comprises a bottom wall part (3212) globally extending according to a bottom plan (wbp) parallel to the base plan (. x/y ) and a lateral wall part (3211) extending along a well lateral direction (wld) disposed according to an angle 0° < a < 180° relatively to the bottom plan (wbp),

wherein the loading opening (325) is defined by a free end of the lateral wall part

(3211) opposite to the bottom plan (wbp), and

wherein:

the bottom wall part (3212) comprises at least one sloped bottom section

(32121),

an inlet port (330) is located off-center in the bottom wall part (3212).

2. A loading well (320) configured to receive a drop of sample (313),

wherein the loading well (320) is an open cavity (324) comprising a wall (321) and a loading opening (325),

wherein the wall (321) comprises a bottom wall part (3212) globally extending according to a bottom plan (wbp) parallel to the base plan (x/y) and a lateral wall part (3211) extending along a well lateral direction (wld) disposed according to an angle 0° < a < 180° relatively to the bottom plan (wbp) and wherein the loading well (320) is at least partially filled with oil,

wherein the loading opening (325) is defined by a free end of the lateral wall part (3211) opposite to the bottom plan (wbp), and

wherein the bottom wall part (3212) comprises at least one sloped bottom section

(32121).

3. The loading well (320) according to any one of claims 1 or 2, wherein the angle a has a value ranging from about 80° to about 105° relatively to the bottom plan (wbp).

4. The loading well (320) according to any one of claims 2 and 3, wherein the oil relative density is greater than 1.01, preferably greater than 1.05, preferably greater than 1.1, preferably greater than 1.5.

5. The loading well (320) according to any one of claims 1 to 4, wherein the inlet port (330) is located:

at a distance diniet-waii in the base plan (x/y) from a curved section of the lateral wall part (3211) ranging from about 0.5 mm to about 2.5 mm; and/or

at a distance in the base plan (x/y) ranging from about 1 mm to about 5 mm from the bottom wall part’s (3212) deepest depth (d) relatively to the loading opening

(325).

6. The loading well (2) according to claim 5, wherein the curved section of the lateral wall part (3211) has a curvature radius ranging from about 0.5 mm to about 2.5 mm, preferably a curvature radius about diniet-waii.

7. A microfluidic chip (300) comprising a loading well (320) according to any one of claims 1 to 6.

8. The micro fluidic chip (300) according to claim 7, further comprising a continuous phase (312), preferably wherein the continuous phase (312) fills partially or completely the micro fluidic network of the micro fluidic chip (300), more preferably wherein the micro fluidic network of the micro fluidic chip (300) comprises at least a droplet generator (340) and a droplet chamber (350).

9. A system for reducing the dead volume of a drop of sample (313) to be loaded in a microfluidic chip (300), the system comprising a loading well (320) according to any one of claims 1 to 6, wherein the system is configured to locate the drop of sample (313) at a di stan ce in the base plan (x/y) rangin g from 0 to 1 diameter of the drop of sample (313).

10. A method for reducing the dead volume of a drop of sample (313) to be loaded in a microfluidic chip (300) comprising an inlet port (330), the method comprising:

- providing the microfluidic chip (300) according to claim 7 or 8,

- placing a drop of sample (313) in the loading well (320),

thereby causing the drop of sample (313) to be located at a distance from the inlet port (330) ranging from 0 to 1 diameter of the drop of sample (313).

11. A system for trapping and/or moving a drop of sample (313) in a well, the system comprising the loading well (320) according to any one of claims 1 to 6, wherein the system is configured to allow the drop of sample (313) to be trapped and/or moved in a defined location of the loading well (320).

12. A method for trapping and/or moving a drop of sample (313) in a well, the method comprising:

- providing a loading well (320) according to any one of claims 1 to 6,

- placing a drop of sample (313) in the loading well (320),

thereby causing the drop of dispersed phase to be trapped and/or moved in a defined location of the loading well (320).