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1. WO2020109143 - MULTIPROTEIN EXPRESSION SYSTEMS FOR PEST CONTROL

Publication Number WO/2020/109143
Publication Date 04.06.2020
International Application No. PCT/EP2019/082152
International Filing Date 21.11.2019
IPC
C07K 19/00 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
19Hybrid peptides
C12N 15/82 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
82for plant cells
CPC
C07K 2319/80
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
2319Fusion polypeptide
80containing a DNA binding domain, e.g. Lacl or Tet-repressor
C12N 15/8286
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
82for plant cells ; , e.g. plant artificial chromosomes (PACs)
8241Phenotypically and genetically modified plants via recombinant DNA technology
8261with agronomic (input) traits, e.g. crop yield
8271for stress resistance, e.g. heavy metal resistance
8279for biotic stress resistance, pathogen resistance, disease resistance
8286for insect resistance
C12N 2800/105
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2800Nucleic acids vectors
10Plasmid DNA
103for invertebrates
105for insects
Applicants
  • FRAUNHOFER-GESELLSCHAFT ZUR FÖRDERUNG DER ANGEWANDTEN FORSCHUNG E.V. [DE]/[DE]
  • JUSTUS-LIEBIG-UNIVERSITÄT GIESSEN [DE]/[DE]
Inventors
  • SCHETELIG, Marc
  • SCHWIRZ, Jonas
  • YAN, Ying
Agents
  • WICHMANN, Hendrik
Priority Data
18208613.227.11.2018EP
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) MULTIPROTEIN EXPRESSION SYSTEMS FOR PEST CONTROL
(FR) SYSTÈMES D'EXPRESSION MULTIPROTÉIQUES DESTINÉS À LA LUTTE CONTRE LES ORGANISMES NUISIBLES
Abstract
(EN)
The application describes a polycistronic expression cassette comprising two or more coding sequences each separated by a coding sequence encoding a self-cleaving peptides sequence. Self-cleaving peptides cause a ribosomal skipping event allowing a polycistronic translation of two or more coding sequences under the control of a single promoter element. One or more of said coding sequences encode one or more lethal gene product(s) making this expression cassette a lethality system. The expression cassette is under the control of a single repressible promoter. The application also describes an expression construct, which further comprises a second expression cassette. The second expression cassette optionally encodes one or more male determining gene(s). Also, the application describes a transgenic insect comprising this polycistronic expression cassette or expression construct. The transgenic insect is a crop pest insect or a disease transmitting insect. The application also describes the use of a transgenic insect according to the invention for controlling reproduction in an insect population of interest. Furthermore, the application provides a method of controlling reproduction in an insect population of interest.
(FR)
L'invention concerne une cassette d'expression polycistronique comprenant deux séquences de codage ou plus, chacune séparée par une séquence de codage codant une séquence peptidique à auto-clivage. Des peptides à auto-clivage provoquent un événement de saut ribosomal permettant une traduction polycistronique d'au moins deux séquences de codage sous la commande d'un élément promoteur unique. Une ou plusieurs desdites séquences de codage codent pour un ou plusieurs produits géniques létaux rendant cette cassette d'expression un système de létalité. La cassette d'expression est sous la commande d'un seul promoteur répressible. L'invention concerne également une construction d'expression, qui comprend en outre une seconde cassette d'expression. La seconde cassette d'expression code éventuellement un ou plusieurs gènes de détermination mâle. L'invention concerne également un insecte transgénique comprenant cette cassette d'expression polycistronique ou une construction d'expression. L'insecte transgénique est un insecte nuisible à la récolte ou un insecte transmettant une maladie. L'invention concerne également l'utilisation d'un insecte transgénique selon l'invention dans la régulation de la reproduction dans une population d'insectes d'intérêt. En outre, l'invention concerne un procédé de régulation de la reproduction dans une population d'insectes d'intérêt.
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