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1. WO2020108757 - MICROBIAL CYTOMETRIC MOCK COMMUNITIES AND USE THEREOF AS STANDARD IN FLOW CYTOMETRY

Publication Number WO/2020/108757
Publication Date 04.06.2020
International Application No. PCT/EP2018/082966
International Filing Date 29.11.2018
IPC
G01N 33/569 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
569for microorganisms, e.g. protozoa, bacteria, viruses
G01N 33/58 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
58involving labelled substances
CPC
G01N 33/569
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
569for microorganisms, e.g. protozoa, bacteria, viruses
G01N 33/582
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
58involving labelled substances
582with fluorescent label
Applicants
  • HELMHOLTZ-ZENTRUM FÜR UMWELTFORSCHUNG GMBH - UFZ [DE]/[DE]
  • LEIBNIZ-INSTITUT DSMZ-DEUTSCHE SAMMLUNG VON MIKROORGANISMEN UND ZELLKULTUREN GMBH [DE]/[DE]
Inventors
  • MÜLLER, Susann
  • CICHOCKI, Nicolas
  • HÜBSCHMANN, Thomas
  • OVERMANN, Jörg
Agents
  • GULDE & PARTNER PATENT- UND RECHTSANWALTSKANZLEI MBB
Priority Data
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) MICROBIAL CYTOMETRIC MOCK COMMUNITIES AND USE THEREOF AS STANDARD IN FLOW CYTOMETRY
(FR) COMMUNAUTÉS FICTIVES CYTOMÉTRIQUES MICROBIENNES ET LEUR UTILISATION EN TANT QU'ÉTALON EN CYTOMÉTRIE DE FLUX
Abstract
(EN)
The present invention is directed to a microbial Cytometric Mock Community for use in flow cytometric analysis, the microbial Cytometric Mock Community comprising or consisting of cells of at least three different microbial species in a pre-defined ratio, wherein the at least three different microbial species are selected such that, when measured using flow cytometry, the specific gate pattern of each microbial species differs significantly from the specific gate pattern of the other microbial species of the microbial Cytometric Mock Community, preferably the at least three different microbial species differ in relative DNA content, relative genomic GC-content, relative cell size, Gram +/- affiliation and/or capacity to form spores. The microbial Cytometric Mock Community shall serve as standardization means that will help ecologists, microbiologists, molecular biologists and flow cytometrists to work on a standardized basis to allow comparison and exchange of data.
(FR)
La présente invention concerne une communauté fictive cytométrique microbienne pour une utilisation en analyse cytométrique de flux, la communauté fictive cytométrique microbienne comprenant ou consistant en des cellules d'au moins trois espèces microbiennes différentes dans un rapport prédéfini, lesdites au moins trois espèces microbiennes différentes étant sélectionnées de telle sorte que, lorsqu'il est mesuré à l'aide d'une cytométrie de flux, le motif de porte spécifique de chaque espèce microbienne diffère significativement du motif de porte spécifique des autres espèces microbiennes de la communauté fictive cytométrique microbienne, de préférence lesdites au moins trois espèces microbiennes différentes diffèrent en termes de contenu d'ADN relatif, de contenu GC génomique relatif, de taille de cellule relative, d'affiliation de Gram +/- et/ou de capacité à former des spores. La communauté fictive cytométrique microbienne servira de moyen de standardisation qui aidera les écologues, les microbiologistes, les biologistes moléculaires et les cytométristes de flux à travailler sur une base standardisée pour permettre la comparaison et l'échange de données.
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