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1. WO2020108582 - REACTION VESSEL, MOLECULAR HYBRIDIZATION APPARATUS, APPLICATION THEREOF, AND DETECTION METHOD

Publication Number WO/2020/108582
Publication Date 04.06.2020
International Application No. PCT/CN2019/121746
International Filing Date 28.11.2019
IPC
G01N 33/536 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
536with immune complex formed in liquid phase
C12M 1/36 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1Apparatus for enzymology or microbiology
36including condition or time responsive control, e.g. automatically controlled fermentors
CPC
C12M 1/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
1Apparatus for enzymology or microbiology
C12M 1/36
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
1Apparatus for enzymology or microbiology
36including condition or time responsive control, e.g. automatically controlled fermentors
C12Q 1/6834
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6813Hybridisation assays
6834Enzymatic or biochemical coupling of nucleic acids to a solid phase
G01N 33/536
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
536with immune complex formed in liquid phase
Applicants
  • 利多(香港)有限公司 LEADWAY (HK) LIMITED [CN]/[CN]
Inventors
  • 张昊 ZHANG, Hao
  • 史亮 SHI, Liang
  • 羊益刚 YANG, Yigang
  • 赵岩 ZHAO, Yan
  • 王志恒 WANG, Zhiheng
  • 朱秀峰 ZHU, Xiufeng
  • 王志海 WANG, Zhihai
  • 缪国超 MIAO, Guochao
  • 谢小恒 XIE, Xiaoheng
Agents
  • 浙江杭州金通专利事务所有限公司 ZHEJIANG HANGZHOU JINTONG PATENT AGENCY CO., LTD.
Priority Data
201811460594.901.12.2018CN
Publication Language Chinese (ZH)
Filing Language Chinese (ZH)
Designated States
Title
(EN) REACTION VESSEL, MOLECULAR HYBRIDIZATION APPARATUS, APPLICATION THEREOF, AND DETECTION METHOD
(FR) CUVE À RÉACTION, APPAREIL D'HYBRIDATION MOLÉCULAIRE, APPLICATION CORRESPONDANTE ET PROCÉDÉ DE DÉTECTION
(ZH) 反应杯、分子杂交仪及其应用和检测方法
Abstract
(EN)
Disclosed is a reaction vessel, comprising a vessel wall and a vessel bottom. The vessel wall is provided with positioning members that allow a membrane strip to stand upright in the reaction vessel. A region on a connecting line between two opposing positioning members constitutes a position in which the membrane strip is placed upright. Also disclosed are a molecular hybridization apparatus for use with the reaction vessel, and a detection method for detecting an analyte. The reaction vessel allows the membrane strip to stand upright therein and to be exposed for operation, so as to reduce the area occupied by the reaction vessel in the molecular hybridization apparatus, thereby reducing the dimensions of the molecular hybridization apparatus. Alternatively, multiple reaction vessels can be placed in the molecular hybridization apparatus at the same time, so as to increase the number of detection operations and enhance the utilization rate and detection efficiency of the molecular hybridization apparatus.
(FR)
L'invention concerne une cuve à réaction, comprenant une paroi de cuve et un fond de cuve. La paroi de cuve comprend des éléments de positionnement qui permettent à une bande de membrane d'être maintenue debout dans la cuve à réaction. Une région sur une ligne de liaison entre deux éléments de positionnement opposés constitue une position dans laquelle la bande de membrane est placée verticalement. L'invention concerne également un appareil d'hybridation moléculaire destiné à être utilisé avec la cuve à réaction, et un procédé de détection permettant de détecter un analyte. La cuve à réaction permet à la bande de membrane de rester verticalement à l'intérieur de cette dernière et d'être exposée à des fins de fonctionnement, de façon à réduire la surface occupée par la cuve à réaction dans l'appareil d'hybridation moléculaire, ce qui permet de réduire les dimensions de l'appareil d'hybridation moléculaire. En variante, de multiples cuves à réaction peuvent être placées dans l'appareil d'hybridation moléculaire en même temps, de façon à augmenter le nombre d'opérations de détection et à améliorer le taux d'utilisation et l'efficacité de détection de l'appareil d'hybridation moléculaire.
(ZH)
一种反应杯,包括杯壁和杯底,杯壁上还设有卡位部,所述卡位部能让膜条竖立于反应杯中,相对的两个卡位部连线的区域形成膜条竖立放置位。本发明还提供了与所述反应杯配合使用的分子杂交仪,以及被分析物检测方法。反应杯可以让膜条竖直放立在其内,并可以敞口操作,节省了反应杯在分子杂交仪所占面积,减少了分子杂交仪的仪器面积。另一方面在分子杂交仪上可以一次性多放置反应杯,增加检测数量,提高分子杂交仪的利用率和工作效率。
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