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1. WO2020108032 - UPLAND COTTON TRANSFORMATION EVENT ICR24-397 AND SPECIFICITY IDENTIFICATION METHOD THEREFOR

Publication Number WO/2020/108032
Publication Date 04.06.2020
International Application No. PCT/CN2019/106639
International Filing Date 19.09.2019
IPC
C12N 15/29 2006.01
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15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
29Genes encoding plant proteins, e.g. thaumatin
C12N 15/82 2006.01
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15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
82for plant cells
A01H 5/00 2018.01
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
HNEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
5Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
CPC
A01H 1/02
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
HNEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
1Processes for modifying genotypes
02Methods or apparatus for hybridisation; Artificial pollination
C12N 15/8261
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15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
82for plant cells ; , e.g. plant artificial chromosomes (PACs)
8241Phenotypically and genetically modified plants via recombinant DNA technology
8261with agronomic (input) traits, e.g. crop yield
C12N 15/8275
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15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
82for plant cells ; , e.g. plant artificial chromosomes (PACs)
8241Phenotypically and genetically modified plants via recombinant DNA technology
8261with agronomic (input) traits, e.g. crop yield
8271for stress resistance, e.g. heavy metal resistance
8274for herbicide resistance
8275Glyphosate
C12Q 1/6895
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QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
6888for detection or identification of organisms
6895for plants, fungi or algae
C12Q 2600/13
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QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
2600Oligonucleotides characterized by their use
13Plant traits
Applicants
  • 中国农业科学院棉花研究所 INSTITUTE OF COTTON RESEARCH, CHINESE ACADEMY OF AGRICULTURAL SCIENCES [CN]/[CN]
Inventors
  • 李付广 LI, Fuguang
  • 葛晓阳 GE, Xiaoyang
  • 王鹏 WANG, Peng
  • 王晔 WANG, Ye
  • 秦文强 QIN, Wenqiang
  • 杨召恩 YANG, Zhaoen
  • 杨作仁 YANG, Zuoren
  • 詹晶晶 ZHAN, Jingjing
  • 鲁丽丽 LU, Lili
  • 张雪妍 ZHANG, Xueyan
  • 王玉芬 WANG, Yufen
Agents
  • 北京纪凯知识产权代理有限公司 JEEKAI & PARTNERS
Priority Data
201811442261.329.11.2018CN
Publication Language Chinese (ZH)
Filing Language Chinese (ZH)
Designated States
Title
(EN) UPLAND COTTON TRANSFORMATION EVENT ICR24-397 AND SPECIFICITY IDENTIFICATION METHOD THEREFOR
(FR) ÉVÉNEMENT DE TRANSFORMATION ICR24-397 DE COTON UPLAND ET PROCÉDÉ D'IDENTIFICATION DE SPÉCIFICITÉ CORRESPONDANT
(ZH) 陆地棉转化事件ICR24-397及其特异性鉴定方法
Abstract
(EN)
Disclosed are an upland cotton transformation event ICR24-397 and a specificity identification method therefor. The method involves: overexpressing the csRRM2 gene in upland cotton CCRI24 to obtain a transgenic cotton ICR24-397, which is cotton obtained by inserting a foreign DNA fragment between the 7000726th and 7000849th positions of chromosome A09 of a target cotton genome to replace the 122bp base sequences between the 7000726th and 7000849th positions of chromosome A09. The experiment proves that the fiber length, the specific strength, the uniformity and the seed cotton yield of the transgenic cotton ICR24-397 are not only higher than that of a receptor material, but the plant height, the leaf length, the leaf width, the number of bolls, the single boll weight, etc., are all also higher than that of the receptor material, which lays a foundation for research of the cultivation of transgenic cotton with good fiber quality.
(FR)
Événement de transformation ICR24-397 de coton Upland et procédé d'identification de spécificité correspondant. Le procédé consiste à surexprimer le gène csRRM2 dans la le coton Upland CCRI24 pour obtenir un coton transgénique ICR24-397, obtenu par insertion d'un fragment d'ADN exogène entre les 7000726 ème et 7000849 ème positions du chromosome a09 d'un génome de coton cible pour remplacer les séquences de bases de 122 (pb) entre les 7000726 ème et 7000849 ème positions du chromosome a09. L'expérience prouve que la longueur de fibre, la résistance spécifique, l'uniformité et le rendement en coton-graine du coton transgénique ICR24-397 sont non seulement supérieurs à celui d'un matériau récepteur, mais la hauteur de plante, la longueur de feuille, la largeur de feuille, le nombre de capsules, le poids de capsule unique, etc., sont tous également supérieurs à celui du matériau de récepteur, qui pose les bases dans la recherche concernant la culture de coton transgénique possédant une bonne qualité de fibre.
(ZH)
陆地棉转化事件ICR24-397及其特异性鉴定方法。在陆地棉CCRI24中过表达csRRM2基因,得到转基因棉花ICR24-397,其为将外源DNA片段插入目的棉花基因组第A09号染色体的第7000726-7000849位间,替换掉第A09号染色体的第7000726-7000849位间122bp的碱基序列后得到的棉花。通过试验证明:转基因棉花ICR24-397不仅纤维长度、比强度、整齐度和籽棉产量高于受体材料,而且株高、叶片长度、叶片宽度、成铃数、单铃重等也均高于受体材料,为培育具有优良纤维品质的转基因棉花的研究奠定基础。
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