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1. WO2020107728 - ZEA MAYS NLP TRANSCRIPTION FACTOR ZMNLP5 AND USE THEREOF

Publication Number WO/2020/107728
Publication Date 04.06.2020
International Application No. PCT/CN2019/075784
International Filing Date 22.02.2019
IPC
C07K 14/415 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
415from plants
C12N 15/82 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
82for plant cells
A01G 22/20 2018.01
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
22Cultivation of specific crops or plants not otherwise provided for
20Cereals
CPC
A01G 22/20
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
22Cultivation of specific crops or plants not otherwise provided for
20Cereals
C07K 14/415
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
415from plants
C12N 15/82
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
82for plant cells ; , e.g. plant artificial chromosomes (PACs)
C12Q 1/6895
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
6888for detection or identification of organisms
6895for plants, fungi or algae
Applicants
  • 江苏省农业科学院 JIANGSU ACADEMY OF AGRICULTURAL SCIENCES [CN]/[CN]
Inventors
  • 葛敏 GE, Min
  • 王元琮 WANG, Yuancong
  • 刘玉河 LIU, Yuhe
  • 赵涵 ZHAO, Han
Agents
  • 上海大视知识产权代理事务所(特殊普通合伙) SHANGHAI DESIGHT INTELLECTUAL PROPERTY AGENCY LLP
Priority Data
201811413552.X26.11.2018CN
Publication Language Chinese (ZH)
Filing Language Chinese (ZH)
Designated States
Title
(EN) ZEA MAYS NLP TRANSCRIPTION FACTOR ZMNLP5 AND USE THEREOF
(FR) FACTEUR DE TRANSCRIPTION ZMNLP5 DE ZEA MAYS NLP ET SON UTILISATION
(ZH) 玉米NLP转录因子ZmNLP5及其应用
Abstract
(EN)
The present invention involves cloning a gene ZmNLP5 from Zea mays that plays an important regulatory role in nitrogen nutrition assimilation and utilization, wherein the open reading frame of the gene has the DNA sequence as shown in SEQ ID NO: 1. A transcription factor protein encoded by the ZmNLP5 gene has the amino acid sequence as shown in SEQ ID NO: 2. Also provided are the uses of the above-mentioned Zea mays NLP transcription factor ZmNLP5 in promoting the expression of a key enzyme gene ZmNIR1.1 for nitrogen metabolism, in promoting the expression of a key enzyme gene ZmNIR1.2 for nitrogen metabolism, in promoting the expression of a key enzyme gene ZmNR1.1 for nitrogen metabolism, in promoting the expression of a key enzyme gene ZmNR1.2 for nitrogen metabolism, in improving the nitrogen nutrition assimilation and utilization by Zea mays, and in promoting the elongation and growth of corn roots in low nitrogen environments. The Zea mays NLP transcription factor ZmNLP5 of the present invention can promote the expression of key enzyme genes for nitrogen metabolism, improve the nitrogen nutrition assimilation and utilization by Zea mays, and promote the elongation and growth of corn roots in low nitrogen environments, and is suitable for large-scale popularization and application.
(FR)
La présente invention concerne le clonage d'un gène ZmNLP5 de Zea mays qui joue un rôle régulateur important dans l'assimilation et l'utilisation de la nutrition azotée, le cadre de lecture ouvert du gène ayant la séquence d'ADN telle que représentée dans SEQ ID NO : 1. Une protéine du facteur de transcription codée par le gène ZmNLP5 a la séquence d'acides aminés telle que représentée dans SEQ ID NO : 2. L'invention concerne également des utilisations du facteur de transcription ZmNLP5 de Zea mays NLP susmentionné dans la promotion de l'expression d'un gène enzymatique clé ZmNIR1.1 pour le métabolisme de l'azote, dans la promotion de l'expression d'un gène enzymatique clé ZmNIR1.2 pour le métabolisme de l'azote, pour favoriser l'expression d'un gène enzymatique clé ZmNR1.1 pour le métabolisme de l'azote, pour favoriser l'expression d'un gène enzymatique clé ZmNR1.2 pour le métabolisme de l'azote, dans l'amélioration de l'assimilation et de l'utilisation de la nutrition azotée par Zea mays, et dans la promotion de l'allongement et de la croissance de racines de maïs dans des environnements à faible teneur en azote. Le facteur de transcription ZmNLP5 de Zea mays NLP selon la présente invention peut favoriser l'expression de gènes enzymatiques clés pour le métabolisme de l'azote, améliorer l'assimilation et l'utilisation de la nutrition azotée par Zea mays, et favoriser l'allongement et la croissance de racines de maïs dans des environnements à faible teneur en azote, et est approprié pour une popularisation et une utilisation à grande échelle.
(ZH)
本发明从玉米中克隆了一个在氮素营养同化利用中发挥重要调控作用的基因ZmNLP5,该基因的开放阅读框具有SEQ ID NO:1所示的DNA序列。ZmNLP5基因所编码的转录因子蛋白具有SEQ ID NO:2所示的氨基酸序列。还提供了上述的玉米NLP转录因子ZmNLP5在促进氮代谢关键酶基因ZmNIR1.1表达中的应用、在促进氮代谢关键酶基因ZmNIR1.2表达中的应用、在促进氮代谢关键酶基因ZmNR1.1表达中的应用、在促进氮代谢关键酶基因ZmNR1.2表达中的应用、在提高玉米氮素营养同化利用中的应用、以及在低氮环境下促进玉米根部伸长生长中的应用。本发明的玉米NLP转录因子ZmNLP5能够促进氮代谢关键酶基因的表达,提高玉米氮素营养同化利用,并在低氮环境下促进玉米根部伸长生长,适于大规模推广应用。
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