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1. WO2020107566 - SMALL-MOLECULE PROTEIN AND USE THEREOF

Publication Number WO/2020/107566
Publication Date 04.06.2020
International Application No. PCT/CN2018/122067
International Filing Date 19.12.2018
IPC
C07K 14/195 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
195from bacteria
C12N 15/867 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
85for animal cells
86Viral vectors
867Retroviral vectors
A61K 49/14 2006.01
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
49Preparations for testing in vivo
06Nuclear magnetic resonance (NMR) contrast preparations; Magnetic resonance imaging (MRI) contrast preparations
08characterised by the carrier
10Organic compounds
14Peptides, e.g. proteins
A61P 39/04 2006.01
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
39General protective or antinoxious agents
04Chelating agents
CPC
A61K 49/14
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
49Preparations for testing in vivo
06Nuclear magnetic resonance [NMR] contrast preparations; Magnetic resonance imaging [MRI] contrast preparations
08characterised by the carrier
10Organic compounds
14Peptides, e.g. proteins
A61P 39/04
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
39General protective or antinoxious agents
04Chelating agents
C07K 14/00
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
C07K 14/195
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
195from bacteria
C12N 15/11
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
C12N 15/867
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
85for animal cells
86Viral vectors
867Retroviral vectors
Applicants
  • 深圳先进技术研究院 SHENZHEN INSTITUTES OF ADVANCED TECHNOLOGY [CN]/[CN]
Inventors
  • 李翔 LI, Xiang
  • 杨海洋 YANG, Haiyang
  • 钱政江 QIAN, Zhengjiang
Agents
  • 北京品源专利代理有限公司 BEYOND ATTORNEYS AT LAW
Priority Data
201811434528.428.11.2018CN
Publication Language Chinese (ZH)
Filing Language Chinese (ZH)
Designated States
Title
(EN) SMALL-MOLECULE PROTEIN AND USE THEREOF
(FR) PROTÉINE À PETITE MOLÉCULE ET UTILISATION CORRESPONDANTE
(ZH) 一种小分子蛋白质及其应用
Abstract
(EN)
Provided are a small-molecule protein and a use thereof, wherein the amino acid sequence of the small-molecule protein is as shown in SEQ ID NO.1; and the small-molecule protein is a specially modified and genetically coded magnetic resonance imaging (MRI) contrast agent. After being expressed in vivo, the gene sequence provided by the present application can significantly shorten the relaxation time of T1 during MRI; and a high signal at relevant sites during imaging solves many problems of MRI contrast agents commonly used at present, such as poor tissue penetration, no cell/tissue selectivity and strong biological toxicity (of metal contrast agents), so that the small-molecule protein can achieve the processes of high-resolution, real-time and in-vivo detection of gene expression, cell differentiation and individual development, etc., and same thus has broad application prospects and a large market value.
(FR)
L'invention concerne une protéine à petite molécule et une utilisation correspondante, la séquence d'acides aminés de la protéine à petite molécule étant telle que présentée dans la séquence SEQ ID NO.1 ; et la protéine à petite molécule est un agent de contraste d'imagerie par résonance magnétique (IRM) spécialement modifié et génétiquement codé. Après avoir été exprimée in vivo, la séquence de gènes fournie par la présente invention peut raccourcir de manière significative le temps de relaxation de T1 pendant l'IRM ; et un signal élevé au niveau de sites pertinents pendant l'imagerie résout de nombreux problèmes d'agents de contraste d'IRM couramment utilisés actuellement, tels qu'une mauvaise pénétration tissulaire, aucune sélectivité cellulaire/tissulaire et une forte toxicité biologique (d'agents de contraste métalliques), de telle sorte que la protéine à petite molécule peut réaliser les processus de détection à haute résolution, en temps réel et in vivo d'expression génique, de différenciation cellulaire et de développement individuel, etc., et elle présente ainsi de larges perspectives d'application et une grande valeur marchande.
(ZH)
提供一种小分子蛋白质及其应用,所述小分子蛋白质的氨基酸序列如SEQ ID NO.1所示;所述小分子蛋白为特殊改造的基因编码的核磁共振成像造影剂;本申请提供的基因序列在生物体内表达后,在核磁成像时能造成T1弛像时间的明显缩短,成像时相关部位高信号,解决了目前常用核磁共振成像造影剂的组织穿透性差、没有细胞/组织选择性、生物毒性强(金属造影剂)等众多问题,能够高分辨率、实时、在体地检测基因表达、细胞分化个体发育过程等,具有广阔的应用前景和巨大的市场价值。
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Latest bibliographic data on file with the International Bureau