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1. WO2020107496 - FLOW CYTOMETRY TESTING METHOD FOR LYMPHOCYTE IN IMMUNE CELL

Publication Number WO/2020/107496
Publication Date 04.06.2020
International Application No. PCT/CN2018/118802
International Filing Date 01.12.2018
IPC
G01N 15/14 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
15Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
10Investigating individual particles
14Electro-optical investigation
G01N 33/577 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
577involving monoclonal antibodies
C12N 5/078 2010.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07Animal cells or tissues
071Vertebrate cells or tissues, e.g. human cells or tissues
078Cells from blood or from the immune system
CPC
G01N 15/14
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
15Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
10Investigating individual particles
14Electro-optical investigation, e.g. flow cytometers
G01N 33/577
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
577involving monoclonal antibodies ; binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens;
Applicants
  • 铭道创新(北京)医疗技术有限公司 MINGDAO INNOVATION(BEIJING)MEDICAL-TECH CO., LTD. [CN]/[CN]
Inventors
  • 姚伟丽 YAO, Weili
Agents
  • 北京市隆安律师事务所 BEIJING LONGAN LAW FIRM
Priority Data
Publication Language Chinese (ZH)
Filing Language Chinese (ZH)
Designated States
Title
(EN) FLOW CYTOMETRY TESTING METHOD FOR LYMPHOCYTE IN IMMUNE CELL
(FR) PROCÉDÉ DE TEST DE CYTOMÉTRIE EN FLUX POUR UN LYMPHOCYTE DANS UNE CELLULE IMMUNITAIRE
(ZH) 一种免疫细胞中淋巴细胞的流式细胞仪检测方法
Abstract
(EN)
Provided is a flow cytometry testing method for a lymphocyte in an immune cell. The method comprises: a) extracting a lymphocyte sample stained with an immunofluorescence antibody, adding same to a pre-chilled PBS-EDTA solution, uniformly mixing the cell, and preparing for a flow cytometry for testing; b) starting up and preheating a flow cytometry system, adjusting the liquid flow velocity, and then adding the PBS-EDTA solution to a sample tube, and washing a flow nozzle system; and c) adding the uniformly mixed cell sample obtained in step a) to the sample tube for testing. The testing method makes subgroup classification more apparent during testing, and analysis and testing more accurate, facilitates testing a small number of cell subgroups, and reduces antibody agents.
(FR)
L'invention concerne un procédé de test de cytométrie en flux pour un lymphocyte dans une cellule immunitaire. Le procédé comprend : a) l'extraction d'un échantillon de lymphocyte coloré avec d'un anticorps d'immunofluorescence, l'ajout de celui-ci à une solution de PBS-EDTA pré-refroidie, le mélange uniforme de la cellule, et la préparation d'une cytométrie en flux pour un test ; b) le démarrage et le préchauffage d'un système de cytométrie en flux, le réglage de la vitesse d'écoulement de liquide, puis l'ajout de la solution de PBS-EDTA à un tube d'échantillon, et le lavage d'un système de buse d'écoulement ; et c) l'ajout de l'échantillon de cellule mélangé uniformément obtenu à l'étape a) au tube d'échantillon pour un test. Le procédé de test rend la classification de sous-groupe plus apparente pendant le test, et l'analyse et le test plus précis, il facilite le test d'un petit nombre de sous-groupes de cellules, et réduit les agents d'anticorps.
(ZH)
提供一种免疫细胞中淋巴细胞的流式细胞仪检测方法,该方法包括:a)取免疫荧光抗体染色的淋巴细胞样品,加入预冷的PBS-EDTA溶液,混均细胞,备流式细胞仪检测待用;b)对流式细胞仪系统进行开机预热,调节液流速度,然后向样品管中加入PBS-EDTA溶液,冲洗液流的喷嘴系统;c)将步骤a)所得混均细胞样品加入样品管然后进行检测。该检测方法在检测时可使亚群分群明显,分析检测更加准确,有利于检测数量较少的细胞亚群,并节省抗体试剂。
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