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1. WO2020097607 - MASSIVELY PARALLEL SEQUENCING USING UNLABELED NUCLEOTIDES

Publication Number WO/2020/097607
Publication Date 14.05.2020
International Application No. PCT/US2019/060779
International Filing Date 11.11.2019
IPC
C12Q 1/6874 2018.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
6869Methods for sequencing
6874involving nucleic acid arrays, e.g. sequencing by hybridisation
C12Q 1/6876 2018.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
Applicants
  • MGI TECH CO., LTD. [CN]/[CN]
  • BGI SHENZEN CO., LTD. [CN]/[CN]
Inventors
  • DRMANAC, Snezana
  • LI, Handong
  • CALLOW, Matthew, J.
  • ECKHARDT, Leon
  • GABLENZ, Scott
  • DRMANAC, Radoje
  • ZHOU, Ping
Agents
  • APPLE, Randolph T.
  • SCHIFF, J. Michael
  • MAO, Yifan
Priority Data
62/758,31709.11.2018US
62/914,87714.10.2019US
62/914,91514.10.2019US
62/914,94014.10.2019US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) MASSIVELY PARALLEL SEQUENCING USING UNLABELED NUCLEOTIDES
(FR) SÉQUENÇAGE MASSIVEMENT PARALLÈLE À L'AIDE DE NUCLÉOTIDES NON MARQUÉS
Abstract
(EN)
The invention provides compositions and methods for sequencing nucleic acids and other applications. In sequencing by synthesis, unlabeled reversible terminators are incorporated by a polymerase in each cycle, then labeled after incorporation by binding to the reversible terminator a directly or indirectly labeled antibody or other affinity reagent.
(FR)
L'invention concerne des compositions et des procédés de séquençage d'acides nucléiques et d'autres applications. Lors d'un séquençage par synthèse, des terminateurs réversibles non marqués sont incorporés par une polymérase dans chaque cycle, puis marqués après incorporation par liaison au terminateur réversible d'un anticorps marqué directement ou indirectement ou d'un autre réactif d'affinité.
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